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Baboon endogenous virus genome: Molecular cloning and structural characterization of nondefective viral genomes from DNA of a baboon cell strain

Cohen, Maurice and Rein, Alan and Stephens, Robert M. and O'Connell, Catherine and Gilden, Raymond V. and Shure, Mavis and Nicolson, Margery O. and McAllister, Robert M. and Davidson, Norman (1981) Baboon endogenous virus genome: Molecular cloning and structural characterization of nondefective viral genomes from DNA of a baboon cell strain. Proceedings of the National Academy of Sciences of the United States of America, 78 (8). pp. 5207-5211. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:COHpnas81

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Abstract

Several heterogeneities in the baboon endogenous virus (BaEV) genomes that are present in the DNA of normal baboon tissues and the baboon cell strain BEF-3 have been described previously. To study these genomes, we cloned BaEV proviruses from BEF-3 cellular DNA into the vector Charon 4A. Of the four full-length clones isolated, one was nondefective as determined by transfection. The sequence of a portion of this clone was found to code for amino acids 61-91 in the p30 region of the gag gene. This identification allowed us to align the restriction map with the BaEV genetic map. One heterogeneity, a BamHI site 2.4 kilobases (kb) from the proviral 5' end, was located close to the gag-pol junction; another, a BamHI site 1.4 kb from the 5' end of the genome, corresponded to the gag p30 coding sequence for amino acids 32-34; and a third, a Xho I site, was near the 3' end of the pol gene. To select the nondefective BaEV genomes from BEF-3 cells, we infected permissive cells with virus produced by BEF-3 cells and also transfected BEF-3 cellular DNA into permissive cells. The BaEV genomes in the permissive recipient cultures were then analyzed by restriction enzyme analysis. These nondefective genomes were found to be heterogeneous with respect to the gag-pol BamHI site and the Xho I site, but all were found to contain the BamHI site 1.4 kb from the 5' end of the genome.


Item Type:Article
Additional Information:© 1981 by the National Academy of Sciences. Contributed by Norman Davidson, April 27, 1981. We thank Ms. Joanne Beheler, Ms. Anne Soria, and Ms. Melody McClure for technical assistance and Ms. Jeannie Clarke and Ms. Margaret Fanning for help in manuscript preparation. This work was supported by Contract N01-CO-75380 with the National Cancer Institute. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.
Subject Keywords:recombinant DNA library; infectious proviruses; DNA sequence determination; genetic map; transfection
Record Number:CaltechAUTHORS:COHpnas81
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:COHpnas81
Alternative URL:http://dx.doi.org/10.1073/pnas.78.8.5207
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7712
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:23 Jul 2007
Last Modified:26 Dec 2012 09:34

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