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Ontogenic activation of a fusion gene introduced into sea urchin eggs

Flytzanis, Constantin N. and Britten, Roy J. and Davidson, Eric H. (1987) Ontogenic activation of a fusion gene introduced into sea urchin eggs. Proceedings of the National Academy of Sciences of the United States of America, 84 (1). pp. 151-155. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:FLYpnas87

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Abstract

Regulatory sequences of a sea urchin cytoskeletal actin gene (CyIIIa) were ligated to the bacterial gene coding for chloramphenicol acetyltransferase (CAT; acetyl-CoA:chloramphenicol O3-acetyltransferase, EC 2.3.1.28) and the construct was injected into unfertilized sea urchin eggs. CAT activity is detected at the early blastula stage, when transcripts of the endogenous CyIIIa gene normally appear. Our measurements show that during activation the amount of CAT enzyme increases at least 100-fold; that there are present in late blastula stage embryos about 5 x 105 molecules of CAT mRNA (i.e., ≈6 times the number of endogenous CyIIIa mRNAs); and that within the range studied the amount of CAT enzyme produced is independent of the number of CyIIIa-CAT genes incorporated per embryo, probably because the genes are present in excess of factors required for their activation. Activation of the CyIIIa-CAT construct is seriously inhibited, or abolished, by successive deletions of upstream CyIIIa sequences.


Item Type:Article
Additional Information:© 1987 by the National Academy of Sciences. Contributed by Eric H. Davidson, September 8, 1986. We are pleased to acknowledge the conscientious and skillful technical assistance of Ms. Lisa Gobar. This work was supported by National Institutes of Health Grant HD-05753. C.N.F. was a Senior Research Fellow of the American Cancer Society, California Division. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.
Subject Keywords:gene transfer; cytoskeletal actin; developmental regulation
Record Number:CaltechAUTHORS:FLYpnas87
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:FLYpnas87
Alternative URL:http://dx.doi.org/10.1073/pnas.84.1.151
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7726
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:23 Jul 2007
Last Modified:26 Dec 2012 09:34

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