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Multiple archaeal groups mediate methane oxidation in anoxic cold seep sediments

Orphan, Victoria J. and House, Christopher H. and Hinrichs, Kai-Uwe and McKeegan, Kevin D. and DeLong, Edward F. (2002) Multiple archaeal groups mediate methane oxidation in anoxic cold seep sediments. Proceedings of the National Academy of Sciences of the United States of America, 99 (11). pp. 7663-7668. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:ORPpnas02

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Abstract

No microorganism capable of anaerobic growth on methane as the sole carbon source has yet been cultivated. Consequently, information about these microbes has been inferred from geochemical and microbiological observations of field samples. Stable isotope analysis of lipid biomarkers and rRNA gene surveys have implicated specific microbes in the anaerobic oxidation of methane (AOM). Here we use combined fluorescent in situ hybridization and secondary ion mass spectrometry analyses, to identify anaerobic methanotrophs in marine methane-seep sediments. The results provide direct evidence for the involvement of at least two distinct archaeal groups (ANME-1 and ANME-2) in AOM at methane seeps. Although both archaeal groups often occurred in direct physical association with bacteria, they also were observed as monospecific aggregations and as single cells. The ANME-1 archaeal group more frequently existed in monospecific aggregations or as single filaments, apparently without a bacterial partner. Bacteria associated with both archaeal groups included, but were not limited to, close relatives of Desulfosarcina species. Isotopic analyses suggest that monospecific archaeal cells and cell aggregates were active in anaerobic methanotrophy, as were multispecies consortia. In total, the data indicate that the microbial species and biotic interactions mediating anaerobic methanotrophy are diverse and complex. The data also clearly show that highly structured ANME-2/Desulfosarcina consortia are not the sole entities responsible for AOM at marine methane seeps. Other microbial groups, including ANME-1 archaea, are capable of anaerobic methane consumption either as single cells, in monospecific aggregates, or in multispecies consortia.


Item Type:Article
Additional Information:Copyright © 2002 by the National Academy of Sciences From the Cover Communicated by John M. Hayes, Woods Hole Oceanographic Institution, Woods Hole, MA, April 6, 2002 (received for review January 23, 2002); Published online before print May 14, 2002, 10.1073/pnas.072210299 We thank Steve Hallam, Chris Preston and Peter Girguis for advice assistance, and Bill Sullivan for use of the University of California Santa Cruz Confocal Microscope Facility. We also thank Tori Hoehler and an anonymous reviewer for helpful comments and suggestions. This work was supported by grants from the David and Lucile Packard Foundation, the Penn State Astrobiology Research Center, and the University of California, Los Angeles, (UCLA) Center for Astrobiology, National Aeronautics and Space Administration National Astrobiology Institute. The UCLA ion microprobe is partially supported by a grant from the National Science Foundation Instrumentation and Facilities Program.
Record Number:CaltechAUTHORS:ORPpnas02
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:ORPpnas02
Alternative URL:http://dx.doi.org/10.1073/pnas.072210299
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:773
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:30 Sep 2005
Last Modified:26 Dec 2012 08:41

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