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Antigen/major histocompatibility complex-specific activation of murine T cells transfected with functionally rearranged T-cell receptor genes

Kuo, Chia-Lam and Hood, Leroy (1987) Antigen/major histocompatibility complex-specific activation of murine T cells transfected with functionally rearranged T-cell receptor genes. Proceedings of the National Academy of Sciences of the United States of America, 84 (21). pp. 7614-7618. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:KUOpnas87

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Abstract

The genes encoding the α and ß chains of the T-cell antigen receptor isolated from a cytochrome c-specific, major histocompatibility complex (MHC)-restricted murine T-cell hybridoma were introduced into a mouse T-cell line of helper lineage by electroporation. In order to examine the contributions of those gene products to antigen and/or MHC specificity, the resultant transfectants were tested for functional antigen and/or MHC recognition. Only those transfectants that express both the introduced genes (α and ß) contributed by the normal T cell can respond specifically to the appropriate antigen/MHC pair. None of the transfectants that express only one of the introduced genes (α or ß) of the normal T cell, or paired hybrid genes (i.e., one gene from the normal T cell and the other from the fusion partner), can respond to the same combination of antigen and MHC product recognized by the donor T cell. However, one clone expressing the transfected genes that encode the α and ß chains of the fusion partner shows reactivity to the antigen-presenting cells even in the absence of the antigens. These data suggest that the αß heterodimer of the T-cell receptor is required to define the fine specificity of a T cell.


Item Type:Article
Additional Information:© 1987 by the National Academy of Sciences. Contributed by Leroy Hood, June 12, 1987. We thank Cheryl Davis for excellent technical assistance; Dr. Nilabh Shastri for helpful advice and stimulating discussion; Dr. Astar Winoto for providing the α-chain cDNAs of V1.9.2 and BW5147 and stimulating discussion; Tim Hunkapiller, Bernard Vernooij, and Drs. Ulf Landegren and Nilabh Shastri for critical comments; and Cathy Elkins for help in preparing the manuscript. The work was supported in part by National Institutes of Health Grant A122274. C.-L.K. is a recipient of an Arthritis Foundation postdoctoral fellowship. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:gene transfer; antigen specificity; major histocompatibiity complex restriction
Record Number:CaltechAUTHORS:KUOpnas87
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:KUOpnas87
Alternative URL:http://www.pnas.org/cgi/content/abstract/84/21/7614
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7731
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:30 Jul 2007
Last Modified:26 Dec 2012 09:34

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