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Immunoreactivity for a calmodulin-dependent protein kinase is selectively increased in macaque striate cortex after monocular deprivation

Hendry, S. H. C. and Kennedy, M. B. (1986) Immunoreactivity for a calmodulin-dependent protein kinase is selectively increased in macaque striate cortex after monocular deprivation. Proceedings of the National Academy of Sciences of the United States of America, 83 (5). pp. 1536-1540. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:HENpnas86

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Abstract

Immunocytochemical methods were used to localize type II Ca2+/calmodulin-dependent protein kinase in the macaque primary visual cortex. Neurons that stain for the kinase include both pyramidal and nonpyramidal cells and they appear to form a subset of cortical neurons. They are densely packed in layers II and IVB, somewhat more sparse in layers III, IVCß, and VI, and nearly absent in layer V. In normal animals the distribution of kinase-positive cells within each layer is relatively uniform. However, in animals in which one eye is removed 7-14 days before sacrifice or sutured shut for 9 or 11 weeks, the cells in layer IVCß are divided into alternating lightly and darkly stained bands. Comparison of immunocytochemically stained sections with adjacent sections stained for the mitochondrial enzyme, cytochrome oxidase, reveals that the kinase staining increases in ocular dominance columns originally driven by the removed or closed eye. These findings suggest that either the concentration of type II Ca2+/calmodulin-dependent protein kinase or its accessibility to the antibody probe increases dramatically and selectively in neurons of macaque primary visual cortex that have been deprived of their normal visual input. This may indicate that changing levels of activity in cortical neurons can alter their regulatory machinery.


Item Type:Article
Additional Information:© 1986 by the National Academy of Sciences. Communicated by Masakazu Konishi, October 23, 1985. We are grateful to Dr. E.G. Jones for his help and guidance in this study and to C. Oto and L. Finger for help with the manuscript. This work was supported by Grant NS10526 from the National Institutes of Health (to Dr. E.G. Jones) and Grant NS17660 to M.B.K. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Record Number:CaltechAUTHORS:HENpnas86
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:HENpnas86
Alternative URL:http://dx.doi.org/10.1073/pnas.83.5.1536
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7753
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:31 Jul 2007
Last Modified:26 Dec 2012 09:34

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