Borkovich, Katherine A. and Alex, Lisa A. and Simon, Melvin I. (1992) Attenuation of Sensory Receptor Signaling by Covalent Modification. Proceedings of the National Academy of Sciences of the United States of America, 89 (15). pp. 6756-6760. ISSN 0027-8424 http://resolver.caltech.edu/CaltechAUTHORS:BORpnas92
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The Tar receptor is a transmembrane protein that regulates bacterial chemotaxis in response to changes in the level of aspartic acid in the medium. The extracellular portion of the protein can bind aspartate, and the cytoplasmic portion modulates CheA kinase activity. The receptor can either activate or inhibit the kinase. The cytoplasmic portion of the receptor can be modified by carboxymethylation of specific glutamic acid residues. To test the effects of differential methylation on receptor function, we prepared membranes from cells that have specifically modified forms of the receptor and tested the relative ability of each of these forms to activate or inhibit CheA kinase. Completely demethylated receptor was a potent inhibitor and poor activator of the kinase, while the fully modified receptor was an excellent activator but an inefficient inhibitor. Partially modified receptor could act both as an effective inhibitor and as an activator. Reversible modification provides a mechanism that allows the cell to accumulate a population of receptor molecules capable of generating a wide range of signaling intensities.
|Additional Information:||Copyright © 1992 by National Academy of Sciences Contributed by Melvin I. Simon, April 24, 1992 We would like to thank R. Stewart for plasmids and B. Bourret, M. Distefano, C. O'Day, A. Pakula, and T. Wilkie for helpful discussions and comments on the manuscript. This work was supported by National Institutes of Health Grants AI-19296 (M.I.S.) and GM-11223 (K.A.B.) and American Cancer Society Grant PF-3516 (L.A.A.). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.|
|Subject Keywords:||chemotaxis, signal transduction, phosphorylation|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Archive Administrator|
|Deposited On:||30 Sep 2005|
|Last Modified:||26 Dec 2012 08:41|
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