Cai, Weiwen and Jing, Junping and Irvin, Benjamin and Ohler, Lynne and Rose, Elise and Shizuya, Hiroaki and Kim, Ung-Jim and Simon, Melvin and Anantharaman, Thomas and Mishra, Bhubaneswar and Schwartz, David C. (1998) High-resolution restriction maps of bacterial artificial chromosomes constructed by optical mapping. Proceedings of the National Academy of Sciences of the United States of America, 95 (7). pp. 3390-3395. ISSN 0027-8424. PMCID PMC19846. http://resolver.caltech.edu/CaltechAUTHORS:CAIpnas98
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Large insert clone libraries have been the primary resource used for the physical mapping of the human genome. Research directions in the genome community now are shifting direction from purely mapping to large-scale sequencing, which in turn, require new standards to be met by physical maps and large insert libraries. Bacterial artificial chromosome libraries offer enormous potential as the chosen substrate for both mapping and sequencing studies. Physical mapping, however, has come under some scrutiny as being "redundant" in the age of large-scale automated sequencing. We report the development and applications of nonelectrophoretic, optical approaches for high-resolution mapping of bacterial artificial chromosome that offer the potential to complement and thereby advance large-scale sequencing projects.
|Additional Information:||Copyright © 1998 by the National Academy of Sciences Contributed by Melvin Simon, January 9, 1998 We thank E. Dimalanta and J. Eddington for experimental assistance. Special thanks go to E. Huff, M. Waterman, M. Urdea, B. Warner, F. Buxton, D. Alexander, and G. Kresbach for helpful discussions. This work was supported by grants from the National Institutes of Health (HG00225-02 and HG00565-03 to E.R.), the National Science Foundation, the W. M. Keck Foundation, and the Lucille P. Markey Charitable Trust. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.|
|Subject Keywords:||genome mapping, fluorescence microscopy, restriction endonuclease, DNA sequencing|
|PubMed Central ID:||PMC19846|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Archive Administrator|
|Deposited On:||30 Sep 2005|
|Last Modified:||19 May 2015 16:19|
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