CaltechAUTHORS
  A Caltech Library Service

Dendritic cell–targeted lentiviral vector immunization uses pseudotransduction and DNA-mediated STING and cGAS activation

Kim, Jocelyn T. and Liu, Yarong and Kulkarni, Rajan P. and Lee, Kevin K. and Dai, Bingbing and Lovely, Geoffrey and Ouyang, Yong and Wang, Pin and Yang, Lili and Baltimore, David (2017) Dendritic cell–targeted lentiviral vector immunization uses pseudotransduction and DNA-mediated STING and cGAS activation. Science Immunology, 2 (13). Art. No. eaal1329. ISSN 2470-9468. http://resolver.caltech.edu/CaltechAUTHORS:20170718-091900989

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20170718-091900989

Abstract

Dendritic cell (DC) activation and antigen presentation are critical for efficient priming of T cell responses. Here, we study how lentiviral vectors (LVs) deliver antigen and activate DCs to generate T cell immunization in vivo. We report that antigenic proteins delivered in vector particles via pseudotransduction were sufficient to stimulate an antigen-specific immune response. The delivery of the viral genome encoding the antigen increased the magnitude of this response in vivo but was irrelevant in vitro. Activation of DCs by LVs was independent of MyD88, TRIF, and MAVS, ruling out an involvement of Toll-like receptor or RIG-I–like receptor signaling. Cellular DNA packaged in LV preparations induced DC activation by the host STING (stimulator of interferon genes) and cGAS (cyclic guanosine monophosphate–adenosine monophosphate synthase) pathway. Envelope-mediated viral fusion also activated DCs in a phosphoinositide 3-kinase–dependent but STING-independent process. Pseudotransduction, transduction, viral fusion, and delivery of cellular DNA collaborate to make the DC-targeted LV preparation an effective immunogen.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/sciimmunol.aal1329DOIArticle
http://immunology.sciencemag.org/content/2/13/eaal1329PublisherArticle
ORCID:
AuthorORCID
Kim, Jocelyn T.0000-0001-8723-8190
Baltimore, David0000-0001-8723-8190
Additional Information:© 2017 American Association for the Advancement of Science.
Record Number:CaltechAUTHORS:20170718-091900989
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20170718-091900989
Official Citation:Dendritic cell–targeted lentiviral vector immunization uses pseudotransduction and DNA-mediated STING and cGAS activation BY JOCELYN T. KIM, YARONG LIU, RAJAN P. KULKARNI, KEVIN K. LEE, BINGBING DAI, GEOFFREY LOVELY, YONG OUYANG, PIN WANG, LILI YANG, DAVID BALTIMORE SCIENCE IMMUNOLOGY21 JUL 2017
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:79147
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:21 Jul 2017 19:52
Last Modified:21 Jul 2017 19:52

Repository Staff Only: item control page