CaltechAUTHORS
  A Caltech Library Service

Biologic activity in a fragment of recombinant human interferon α

Ackerman, Samuel K. and Zur Nedden, Dorothy and Heintzelman, Mark and Hunkapiller, Michael and Zoon, Kathryn (1984) Biologic activity in a fragment of recombinant human interferon α. Proceedings of the National Academy of Sciences of the United States of America, 81 (4). pp. 1045-1047. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:ACKpnas84

[img]
Preview
PDF
See Usage Policy.

757Kb

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:ACKpnas84

Abstract

To attempt to locate functionally important regions of the interferon (IFN) molecule, recombinant human IFN-α2 was subjected to proteolytic digestion. The bacterial proteinase thermolysin produced two major complementary fragments, HuIFN-α2-(1-110) and HuIFN-α2-(111-153). After reduction with 2-mercaptoethanol and separation of the two major fragments on NaDodSO4/polyacrylamide gel electrophoresis, antiviral activity persisted in the larger, Mr 12,000, fragment consisting of the amino-terminal 110 amino acids.


Item Type:Article
Additional Information:© 1984 by the National Academy of Sciences. Communicated by Christian B. Anfinsen, November 2, 1983. We thank Dr. N.-Y. Nguyen for amino acid analysis and Dr. R. Wetzel for helpful discussion. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:thermolysin; molecular sizes; partial amino acid sequence; antiviral activity
Record Number:CaltechAUTHORS:ACKpnas84
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:ACKpnas84
Alternative URL:http://www.pnas.org/cgi/content/abstract/81/4/1045
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8086
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:30 Jul 2007
Last Modified:26 Dec 2012 09:36

Repository Staff Only: item control page