Zuñiga, Martha C. and D'Eustachio, Peter and Ruddle, Nancy H. (1982) Immunoglobulin heavy chain gene rearrangement and transcription in murine T cell hybrids and T lymphomas. Proceedings of the National Academy of Sciences of the United States of America, 79 (9). pp. 3015-3019. ISSN 0027-8424 http://resolver.caltech.edu/CaltechAUTHORS:ZUNpnas82
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We have examined the arrangement of immunoglobulin heavy chain constant (CH) and joining (JH) region genes in murine T cell hybrid lines and in T lymphomas. CH genes derived from both parental cell types were present in all hybrids for which polymorphism in sequences flanking CH genes permitted us to distinguish parental CH genes. All T lymphomas and T cell hybrids retained the Cα gene in germ-line configuration and all but one cell line had germ-line Cµ genes. Novel DNA fragments reactive with JH probes were observed in six of nine T cell hybrids, as well as in two T lymphomas, WEH17.1 and YAC-1, but not in the fusion parent, BW5147. No RNA homologous to Cγ2b, Cα, or λ genes was detected in any of the T cell lines. T cell lines contained poly(A)+ RNA homologous to a Cµ cDNA probe. More importantly, in several cell lines the Cµ RNAs were associated with membrane-bound polyribosomes. These results suggest that both JH rearrangements and Cµ RNA production occur in at least some mature, antigen-specific T cells. They may therefore reflect events in normal T cell development and function related to those involved in the generation of the T receptor for antigen.
|Additional Information:||© 1982 by the National Academy of Sciences. Communicated by Frank H. Ruddle, February 1, 1982. We thank Belinda Beezley for technical assistance, Tim Hunkapillar for the computer analysis of the mouse μ gene, and Ann Rothstein and Steve Carson for cell lines. We thank Michael Ernest for suggesting the RNA isolation procedure and Ken Marcu and Frank Ruddle for experimental material and helpful discussions. This work was supported by National Institutes of Health Grants CA16885 and GM09966. M.C.Z. was supported by National Research Service Award 1 T32 AI-07098 from the National Institutes of Health. P.D.E. was a Leukemia Society of America Special Fellow. N.H.R. was a recipient of an American Cancer Society Faculty Research Award, ACS-FRA-196. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.|
|Subject Keywords:||immunoglobulin gene polymorphism; polysomal mRNA|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Tony Diaz|
|Deposited On:||01 Aug 2007|
|Last Modified:||26 Dec 2012 09:36|
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