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DNA-binding properties of Arabidopsis MADS domain homeotic proteins APETALA1, APETALA3, PISTILLATA and AGAMOUS

Riechmann, José Luis and Wang, Minqin and Meyerowitz, Elliot M. (1996) DNA-binding properties of Arabidopsis MADS domain homeotic proteins APETALA1, APETALA3, PISTILLATA and AGAMOUS. Nucleic Acids Research, 24 (16). pp. 3134-3142. ISSN 0305-1048. PMCID PMC146081. http://resolver.caltech.edu/CaltechAUTHORS:RIEnar96

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Abstract

The MADS domain proteins APETALA1 (AP1), APETALA3 (AP3), PISTILLATA (PI), and AGAMOUS (AG) specify the identity of Arabidopsis floral organs. AP1 and AG homocomplexes and AP3-PI heterocomplexes bind to CArG-box sequences. The DNA-binding properties of these complexes were investigated. We find that AP1, AG and AP3-PI are all capable of recognizing the same DNA-binding sites, although with somewhat different affinities. In addition, the three complexes induce similar conformational changes on a CArG-box sequence. Phasing analysis reveals that the induced distortion is DNA bending, oriented toward the minor groove. The molecular dissection of AP1, AP3, PI and AG indicates that the boundaries of the dimerization domains of these proteins vary. The regions required to form a DNA-binding complex include, in addition to the MADS box, the entire L region (which follows the MADS box) and the first putative amphipathic helix of the K box in the case of AP3-PI, while for AP1 and AG only a part of the L region is needed. The similarity of the DNA-binding properties of AP1, AP3-PI and AG is discussed with regard to the biological specificity that these proteins exhibit.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1093/nar/24.16.3134DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC146081/PubMed CentralArticle
ORCID:
AuthorORCID
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 1996 Oxford University Press. Received May 28, 1996; Accepted July 3, 1996. We are grateful to Hong Ma for providing binding site probes, to Sankar Adhya for pBend2, and to members of the laboratory for valuable comments on the manuscript. This work was supported by US National Science Foundation grant MCB-9204839 to E.M.M.; J.L.R. was supported by a fellowship from Ministerio de Educación y Ciencia (Spain) and was formerly an EMBO Postdoctoral Fellow.
Funders:
Funding AgencyGrant Number
NSFMCB-9204839
Ministerio de Educación y Ciencia (MEC)UNSPECIFIED
European Molecular Biology Organization (EMBO)UNSPECIFIED
PubMed Central ID:PMC146081
Record Number:CaltechAUTHORS:RIEnar96
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:RIEnar96
Alternative URL:http://dx.doi.org/10.1093/nar/24.16.3134
Official Citation:José Luis Riechmann, Minqin Wang, and Elliot M. Meyerowitz DNA-Binding Properties of Arabidopsis MADS Domain Homeotic Proteins APETALA1, APETALA3, PISTILLATA and AGAMOUS Nucl. Acids Res. (1996) 24 (16): 3134-3141 doi:10.1093/nar/24.16.3134
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ID Code:821
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:08 Oct 2005
Last Modified:10 Dec 2016 18:31

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