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Single-base mismatch detection based on charge transduction through DNA

Kelley, Shana O. and Boon, Elizabeth M. and Barton, Jacqueline K. and Jackson, Nicole M. and Hill, Michael G. (1999) Single-base mismatch detection based on charge transduction through DNA. Nucleic Acids Research, 27 (24). pp. 4830-4837. ISSN 0305-1048. PMCID PMC148785. http://resolver.caltech.edu/CaltechAUTHORS:KELnar99

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Abstract

High-throughput DNA sensors capable of detecting single-base mismatches are required for the routine screening of genetic mutations and disease. A new strategy for the electrochemical detection of single-base mismatches in DNA has been developed based upon charge transport through DNA films. Double-helical DNA films on gold surfaces have been prepared and used to detect DNA mismatches electrochemically. The signals obtained from redox-active intercalators bound to DNA-modified gold surfaces display a marked sensitivity to the presence of base mismatches within the immobilized duplexes. Differential mismatch detection was accomplished irrespective of DNA sequence composition and mismatch identity. Single-base changes in sequences hybridized at the electrode surface are also detected accurately. Coupling the redox reactions of intercalated species to electrocatalytic processes in solution considerably increases the sensitivity of this assay. Reporting on the electronic structure of DNA, as opposed to the hybridization energetics of single-stranded oligonucleotides, electrochemical sensors based on charge transport may offer fundamental advantages in both scope and sensitivity.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC148785/PubMed CentralArticle
ORCID:
AuthorORCID
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© Oxford University Press, 1999. Received April 7, 1999; Revised September 15, 1999; Accepted October 1, 1999. We wish to thank Professor F. A. Anson for several helpful discussions. This work was supported by the Camile and Henry Dreyfus Foundation (Faculty Start-up Grant to M.G.H.), the Research Corporation (M.G.H.), and the National Institutes of Health (GM49216 to J.K.B.).
Funders:
Funding AgencyGrant Number
Camille and Henry Dreyfus FoundationUNSPECIFIED
Research CorporationUNSPECIFIED
NIHGM49216
PubMed Central ID:PMC148785
Record Number:CaltechAUTHORS:KELnar99
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:KELnar99
Alternative URL:http://dx.doi.org/10.1093/nar/27.24.4830
Official Citation:Shana O. Kelley, Elizabeth M. Boon, Jacqueline K. Barton, Nicole M. Jackson, and Michael G. Hill Single-base mismatch detection based on charge transduction through DNA Nucl. Acids Res. (1999) 27 (24): 4830-4837 doi:10.1093/nar/27.24.4830
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:822
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:08 Oct 2005
Last Modified:26 Jan 2016 04:04

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