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Cotransfer of the Eαd and Aβd genes into L cells results in the surface expression of a functional mixed-isotype Ia molecule

Malissen, Bernard and Shastri, Nilabh and Pierres, Michel and Hood, Leroy (1986) Cotransfer of the Eαd and Aβd genes into L cells results in the surface expression of a functional mixed-isotype Ia molecule. Proceedings of the National Academy of Sciences of the United States of America, 83 (11). pp. 3958-3962. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:MALpnas86

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Abstract

Ia molecules play a key role in antigen recognition by T lymphocytes. To analyze the structural features of the individual α and β chains relevant to the assembly of intact Ia molecules, mouse fibroblasts were contransfected with various combinations of haplotype- and isotype-mismatched Ia α/β gene pairs. Two important points emerged. First, the level of surface expression of a given haplotype-mismatched AαAβ pair appears to depend upon the α and β chain alleles involved. Second, transfection with some isotype-mismatched combinations such as EαdAβd results in a significant level of surface expression of a stable mixed-isotype dimer, which also appears to be normally expressed at a low level by an Iad-positive B lymphoma. Moreover, a T-cell hybridoma specific for human gamma globulin and restricted by the Ed molecule was found to be efficiently stimulated by the EαdAβd-positive transfectant in the presence of antigen. The stimulation was specifically inhibited by monoclonal antibodies directed to either the Ia or the L3T4 molecule. These findings suggest that the estimates of the potential number of Ia molecules available in an animal for restricting T-lymphocyte recognition of antigens must be revised.


Item Type:Article
Additional Information:© 1986 by the National Academy of Sciences. Contributed by Leroy Hood, January 21, 1986. We thank Drs. Philippa Marrack and Gunter Hammerling for the AODH7.1 hybridoma and the anti-Ad monoclonal antibodies, Ms. Sylvie Marchetto and Deborah Munt for expert technical assistance, and Ms. Colette Bellegarde-Kourilsky and Connie Katz for typing this manuscript. This work was supported by institutional grants from Institut National de la Santé et de la Recherche Médicale, Centre National de la Recherche Scientifique, and National Institutes of Health. N.S. is a Lievre Fellow of the California Division of the American Cancer Society. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:gene transfer; Ia molecule; α/β chain gene pairing; T-cell recognition; immune response genes
Record Number:CaltechAUTHORS:MALpnas86
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:MALpnas86
Alternative URL:http://www.pnas.org/cgi/content/abstract/83/11/3958
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8448
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:14 Aug 2007
Last Modified:14 Nov 2014 19:20

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