Makino, Clint L. and Dodd, R. L. and Chen, J. and Burns, M. E. and Roca, A. and Simon, M. I. and Baylor, D. A. (2004) Recoverin Regulates Light-dependent Phosphodiesterase Activity in Retinal Rods. Journal of General Physiology, 123 (6). pp. 729-741. ISSN 0022-1295 http://resolver.caltech.edu/CaltechAUTHORS:MAKjgp04
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The Ca2+-binding protein recoverin may regulate visual transduction in retinal rods and cones, but its functional role and mechanism of action remain controversial. We compared the photoresponses of rods from control mice and from mice in which the recoverin gene was knocked out. Our analysis indicates that Ca2+-recoverin prolongs the dark-adapted flash response and increases the rod's sensitivity to dim steady light. Knockout rods had faster Ca2+ dynamics, indicating that recoverin is a significant Ca2+ buffer in the outer segment, but incorporation of exogenous buffer did not restore wild-type behavior. We infer that Ca2+-recoverin potentiates light-triggered phosphodiesterase activity, probably by effectively prolonging the catalytic activity of photoexcited rhodopsin.
|Additional Information:||© 2004 The Rockefeller University Press Submitted: 16 December 2003. Accepted: 3 May 2004. Published online June 1 2004. doi:10.1085/jgp.200308994 We thank Drs. V. Arshavsky, L. Donoso, P. Hargrave, J. Hurley, R. Lefkowitz, and K. Palczewski for generously providing us with antibodies, Mr. S. Nagpal for the microchip analyses, Ms. C. Mfalila for assistance with the statistical analysis, and Mr. R. Schneeveis for skillful, technical support. This research was funded by a Career Development Award from Research to Prevent Blindness (C.L. Makino), the Arnold and Mabel Beckman Foundation (J. Chen), the National Institute of General Medical Sciences (R.L. Dodd), the National Institute on Aging AG12288 (M.I. Simon), and the National Eye Institute EY12944 (C.L. Makino), EY12703 (J. Chen), EY14047 (M.E. Burns), EY05750 (D.A. Baylor). Correction for Makino et al., J. Gen. Physiol. 123 (6) 729-741. Published online Jun 27 2005. doi:10.1085/jgp.200308994061405c|
|Subject Keywords:||calcium-binding proteins, knockout mice, light adaptation, photoreceptors, phototransduction|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Archive Administrator|
|Deposited On:||20 Aug 2007|
|Last Modified:||26 Dec 2012 09:40|
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