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The process of infection with bacteriophage phiX174, XXII. Synthesis of progeny single-stranded DNA

Komano, Tohru and Knippers, Rolf and Sinsheimer, Robert L. (1968) The process of infection with bacteriophage phiX174, XXII. Synthesis of progeny single-stranded DNA. Proceedings of the National Academy of Sciences of the United States of America, 59 (3). pp. 911-916. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:KOMpnas68

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Abstract

In a previous article,(1) it was demonstrated that of the labeled thymidine incorporated into the replicative form (RF) of phiX-DNA during the period of RF replication (3-12 or 15 min after infection), approximately half was transferred to virus during the subsequent period of single-strand DNA synthesis, while half remained in RF molecules. In this paper experiments are presented which permit a definition of the label remaining in the RF and which clarify the role of the RF in the synthesis of the progeny single-stranded DNA.


Item Type:Article
Additional Information:© 1968 by the National Academy of Sciences. Communicated December 20, 1967. We are indebted to Dr. M. Goulian for a generous gift of purified exonuclease III and to Mrs. Louise Wilson for technical assistance. This research was supported in part by grant GM 13554 from the U.S. Public Health Service.
Record Number:CaltechAUTHORS:KOMpnas68
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:KOMpnas68
Alternative URL:http://www.pnas.org/cgi/reprint/59/3/911
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8592
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:21 Aug 2007
Last Modified:26 Dec 2012 09:40

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