Pikó, Lajos and Hammons, Michele D. and Taylor, Kent D. (1984) Amounts, synthesis, and some properties of intracisternal A particle-related RNA in early mouse embryos. Proceedings of the National Academy of Sciences of the United States of America, 81 (2). pp. 488-492. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:PIKpnas84
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Early mouse embryos express two morphological subtypes of intracisternal A-type particles, one resembling those occurring in mouse tumors (referred to as IAP) and the other apparently specific for early embryos [referred to as IAP(ε)]. Using cloned fragments of IAP genes as labeled probes in dot-hybridization experiments, we detected IAP-related RNA sequences in mouse oocytes and preimplantation embryos. IAP RNA is relatively abundant in ovarian oocytes, is reduced in amount to ≈1/10th in the ovulated egg, and increases ≈100 times (from ≈1.3x10^(3) to ≈1.5x10^(5) molecules per embryo) between the one-cell stage and late blastocyst stage. Most of the IAP RNA consists of a single size class of about 5.4 kilobases, and a major fraction of this RNA is polyadenylylated. Quantitative considerations suggest that only a few percent of the IAP RNA in embryos are associated with particles. In two-cell embryos, the number of IAP RNA molecules is <1/10th the number of IAP(ε) particles, suggesting that IAP(ε) is genetically distinct from IAP and presumably represents a family of as yet unidentified retrovirus-like elements.
|Additional Information:||© 1984 by the National Academy of Sciences. Communicated by Roy J. Britten, September 30, 1983. We thank Mary Ann Chie for excellent technical assistance. We are indebted to Mark Davis for his help in screening the mouse genomic library. This work was supported by the Medical Research Service of the Veterans Administration and by U.S. Public Health Service Grant CA24989 from the National Cancer Institute. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.|
|Subject Keywords:||endogenous retrovirus; gene expression; early development; RNA-DNA hybridization|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Tony Diaz|
|Deposited On:||22 Aug 2007|
|Last Modified:||14 Nov 2014 19:20|
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