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The distribution of buoyant density of human erythrocytes in bovine albumin solutions

Leif, Robert C. and Vinograd, Jerome (1964) The distribution of buoyant density of human erythrocytes in bovine albumin solutions. Proceedings of the National Academy of Sciences of the United States of America, 51 (3). pp. 520-528. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:LEIpnas64

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Abstract

Several reports have appeared in the literature of the variation of "density" among different types of dispersed cells and of physical separations based upon this property. (1,2) Two general separation procedures have been used. Packed cell methods: cells are centrifuged from a suspension to form a viscous mass of packed cells, which is separated layerwise. Neutral density separations: cells are centrifuged in a dense liquid medium and are segregated into two fractions, one denser and one lighter than the suspending medium. With such methods the order of density of certain types of blood cells has been established. (3-5) It has also been shown that erythrocytes are heterogeneous in density and that young erythrocytes are less dense than old. (6-9)


Item Type:Article
Additional Information:© 1964 by the National Academy of Sciences. Communicated by Norman Davidson, January 2, 1964. It is a pleasure to thank C. M. Pomerat for permission to use the Model B Coulter counter at the Pasadena Foundation for Medical Research, L. Capers and D. Hammond for the cytological studies, and D. Kabat and G. Attardi for permission to cite their unpublished results. This work was supported in part by grant HE 03394 from the USPHS. Gates and Crellin Laboratories of Chemistry, Contribution no. 3045.
Record Number:CaltechAUTHORS:LEIpnas64
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:LEIpnas64
Alternative URL:http://www.pnas.org/cgi/reprint/51/3/520
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8621
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:31 Aug 2007
Last Modified:26 Dec 2012 09:40

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