Lee, Amy Shiu and Thomas, Terry L. and Lev, Ze'ev and Britten, Roy J. and Davidson, Eric H. (1980) Four sizes of transcript produced by a single sea urchin gene expressed in early embryos. Proceedings of the National Academy of Sciences of the United States of America, 77 (6). pp. 3259-3263. ISSN 0027-8424. PMCID PMC349594. http://resolver.caltech.edu/CaltechAUTHORS:LEEpnas80
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This report concerns a set of sea urchin egg and embryo transcripts complementary to a single-copy region of a cloned DNA fragment (Sp88). Three distinct 16-cell embryo polysomal RNA species were found to hybridize with this fragment. These RNAs are about 1700, 3000, and 4000 nucleotides (nt) in length, and the same species were identified in unfertilized eggs. A significant fraction of all three species of the egg and early embryo transcripts is polyadenylylated. At gastrula stage Sp88 transcripts are almost completely confined to the nucleus [Lev, Z., Thomas, T. L., Lee, A. S., Angerer, R. C., Britten, R. J. & Davidson, E. H. (1980) Dev. Biol. 75, in press]. The Sp88 transcripts of gastrulae are present as a fourth RNA species approximately 5800 nt in length. The four species share a sequence element of the cloned DNA fragment that is about 1000 nt long. These RNAs constitute a set of alternative partially overlapping transcripts from the same genomic region.
|Additional Information:||© 1980 by the National Academy of Sciences. Contributed by Roy J. Britten, March 14, 1980. This research was supported by National Institutes of Health Grant HD-05753. A.S.L. was supported by an American Cancer Society, California Division, Senior Fellowship (D-353). T.L.T. was supported by an ACS, California Division, Lievre Fellowship (J-409). Z.L. was supported by a Chaim Weizmann Fellowship and by a Gosney Fellowship. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.|
|Subject Keywords:||maternal messenger RNA; nuclear RNA; cloned gene|
|PubMed Central ID:||PMC349594|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Tony Diaz|
|Deposited On:||31 Aug 2007|
|Last Modified:||18 Dec 2015 01:51|
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