Forsheit, Arleen B. and Ray, Dan S. (1970) Conformations of the single-stranded DNA of bacteriophage M13. Proceedings of the National Academy of Sciences of the United States of America, 67 (3). pp. 1534-1541. ISSN 0027-8424 http://resolver.caltech.edu/CaltechAUTHORS:FORpnas70
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At least two conformations of M13 single-stranded DNA have been demonstrated by measuring differences in sedimentation coefficient and by direct visualization in the electron microscope. Which form is obtained from infected cells and/or intact phage depends on the pH, ionic strength, and temperature. The slower-sedimenting form can be converted to the faster-sedimenting, single-stranded form by low ionic strength, alkali treatment, formamide, or formaldehyde, but not by exposure to 100 degrees C in 1.0 M NaCl. The ability to assume either conformation appears to be a function of the nucleic acid alone. Whether or not these different conformations are of biological significance is still unknown.
|Additional Information:||© 1970 by the National Academy of Sciences. Communicated by Norman Davidson, August 28, 1970. Supported by NSF grant GB 18074, University of California Cancer Research Funds, and an NSF predoctoral traineeship to A.B.F.|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Tony Diaz|
|Deposited On:||06 Sep 2007|
|Last Modified:||26 Dec 2012 09:41|
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