Lunardi, Joël and Hurko, Orest and Engel, W. King and Attardi, Giuseppe (1992) The multiple ADP/ATP translocase genes are differentially expressed during human muscle development. Journal of Biological Chemistry, 267 (22). pp. 15267-15270. ISSN 0021-9258 http://resolver.caltech.edu/CaltechAUTHORS:LUNjbc92
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The expression of the genes encoding the three isoforms of the human ADP/ATP translocase (T1, T2, and T3) has been analyzed at different stages of myogenic differentiation in an in vitro muscle cell system and compared with that in mature muscle. The results indicate that the three stages of muscle differentiation corresponding to myoblast proliferation, myotube formation, and mature muscle fibers are characterized by a different pattern of expression of the ADP/ATP translocase genes. In particular, the two T2-specific mRNAs are present at high, similar levels in myoblasts and myotubes and markedly decrease in amount in mature adult muscle. By contrast, the T3-specific mRNA is present in high amount in growing myoblasts, decreases markedly in myotubes, and is barely detectable in adult muscle. Finally, the T1-specific mRNA is present at a high level in adult muscle and is not detectable in either myoblasts or myotubes. Therefore, T1 gene expression appears to be a marker of a late stage in myogenesis. A parallel investigation of expression of the myosin heavy chain mRNA revealed absence of hybridization with the specific probe in RNA from proliferating myoblasts, a significant hybridization in myotube RNA, and a strong signal in adult muscle RNA.
|Additional Information:||Copyright © 1992 by the American Society for Biochemistry and Molecular Biology. (Received for publication, March 23, 1992) We thank Dr. H. Weintraub for the gift of the MHC clone. We gratefully acknowledge the technical assistance of Arger Drew, Linda McKee, Benneta Keeley, and Lisa Tefo. These investigations were supported by National Institutes of Health Grants GM-11726 (to G.A.) and AR38231 (to O.H.), an Eleanor Roosevelt-International Union against Cancer Fellowship, and a grant from the Association Francaise Contre les Myopathies (to J.L.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.|
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|Deposited On:||11 Sep 2007|
|Last Modified:||26 Dec 2012 09:41|
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