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Stereoselective hydroxylation of an achiral cyclopentanecarboxylic acid derivative using engineered P450s BM-3

Münzer, Dieter F. and Meinhold, Peter and Peters, Matthew W. and Feichtenhofer, Sabine and Griengl, Herfried and Arnold, Frances H. and Glieder, Anton and de Raadt, Anna (2005) Stereoselective hydroxylation of an achiral cyclopentanecarboxylic acid derivative using engineered P450s BM-3. Chemical Communications, 2005 (20). pp. 2597-2599. ISSN 1359-7345. http://resolver.caltech.edu/CaltechAUTHORS:MUNcc05

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Abstract

Substrate engineered, achiral carboxylic acid derivative 2 was biohydroxylated with various mutants of cytochrome P450 BM-3 to give two out of the four possible diastereoisomers in high de and ee. The BM-3 mutants exhibit up to 9200 total turnovers for hydroxylation of the engineered substrate, which without the protecting group is not transformed by this enzyme.


Item Type:Article
Additional Information:This journal is © The Royal Society of Chemistry 2005. Received (in Cambridge, UK) 2nd February 2005, Accepted 21st March 2005. First published on the web 6th April 2005. Electronic supplementary information (ESI) available: experimental details. See http://www.rsc.org/suppdata/cc/b5/b501527h/
Record Number:CaltechAUTHORS:MUNcc05
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:MUNcc05
Alternative URL:http://dx.doi.org/10.1039/b501527h
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8763
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:13 Sep 2007
Last Modified:26 Dec 2012 09:42

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