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Spectroscopic studies of wild-type and mutant "zinc finger" peptides: Determinants of domain folding and structure

Párraga, Grace and Horvath, Suzanne and Hood, Leroy and Young, E. T. and Klevit, Rachel E. (1990) Spectroscopic studies of wild-type and mutant "zinc finger" peptides: Determinants of domain folding and structure. Proceedings of the National Academy of Sciences of the United States of America, 87 (1). pp. 137-141. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:PARpnas90

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Abstract

The "zinc finger" model [Miller, J., McLachlan, A.D. & Klug, A. (1985) EMBO J. 4, 1609-1614; Brown, R. S., Sander, C. & Argos, P. (1985) FEBS Lett. 186, 271-274] makes both specific structural and specific functional predictions about zinc finger consensus sequences that can be tested with a combination of genetic, molecular biological, and biophysical techniques. The yeast transcription factor ADR1 contains two adjacent zinc finger domains; genetic and deletion analyses showed that amino acid substitutions and deletions in the zinc finger domains resulted in the loss of protein activity. To test the structural and folding predictions of the zinc finger model, peptides encompassing each of the ADR1 fingers were synthesized (ADR1a and ADR1b) as well as a mutant finger peptide (del138) deleted for a single amino acid residue. The folding and metal-binding characteristics of these were assessed by 1H nuclear magnetic resonance (NMR) and visible spectros-copy. While a single unique conformational species was detected for the two wild-type peptides upon tetrahedral binding of zinc, the deletion peptide did not bind zinc with tetrahedral geometry, nor did it fold into a zinc finger domain. The metal-binding and folding results found with the mutant peptide were similar to those obtained when thiol alkylation or imidazole protonation of the wild-type peptides was performed. These data indicate that ligand spacing and both thiol and imidazole participation in zinc binding are specific and necessary requirements for zinc finger folding, which provides direct support for the initial predictions of the model.


Item Type:Article
Additional Information:© 1990 by the National Academy of Sciences. Contributed by Leroy Hood, October 3, 1989. The authors are grateful for peptide sequencing and time-of-flight and fast atom bombardment mass spectroscopy performed with the assistance of Lowell Ericsson, Harry Charbonneau, and Ken Walsh (Dept. of Biochemistry, University of Washington). We also thank Gary Drobny and Brian Reid for use of NMR facilities (Dept. of Chemistry, University of Washington). We especially acknowledge our co-workers Melissa Starovasnik, Michael Wittekind, and Jon Herriott, as well as Colin Manoil (Dept. of Genetics, University of Washington) and Dave Teller, for their careful reading of the manuscript. This work was supported by National Institutes of Health Grant 2 P01 32681 to R.E.K. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:nuclear magnetic resonance; transcription factor ADR1
Record Number:CaltechAUTHORS:PARpnas90
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:PARpnas90
Alternative URL:http://www.pnas.org/cgi/content/abstract/87/1/137
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8789
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:17 Sep 2007
Last Modified:14 Nov 2014 19:20

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