CaltechAUTHORS
  A Caltech Library Service

Cyclic polyamides for recognition in the minor groove of DNA

Cho, Junhyeong and Parks, Michelle E. and Dervan, Peter B. (1995) Cyclic polyamides for recognition in the minor groove of DNA. Proceedings of the National Academy of Sciences of the United States of America, 92 (22). pp. 10389-10392. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:CHOpnas95

[img]
Preview
PDF
See Usage Policy.

981Kb

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:CHOpnas95

Abstract

Small molecules that specifically bind with high affinity to any designated DNA sequence in the human genome would be useful tools in molecular biology and potentially in human medicine. Simple rules have been developed to rationally alter the sequence specificity of minor groove-binding polyamides containing N-methylimidazole and N-methylpyrrole amino acids. Crescent-shaped polyamides bind as antiparallel dimers with each polyamide making specific contacts with each strand on the floor of the minor groove. Cyclic polyamides have now been synthesized that bind designated DNA sequences at subnanomolar concentrations.


Item Type:Article
Additional Information:Copyright © 1995 by the National Academy of Sciences. Contributed by Peter B. Dervan, July 21, 1995. We thank the Mass Spectrometry Laboratory (University of California, Riverside) for the mass spectral analysis. We are grateful to the National Institutes of Health (Grant 27681) for research support and a National Institutes of Health Research Service Award to M.E.P.
Subject Keywords:SEQUENCE-SPECIFIC RECOGNITION, COVALENT PEPTIDE DIMERS, FOOTPRINT TITRATION, 2-DIMENSIONAL NMR, DISTAMYCIN-A, MOTIF, BINDING, COMPLEX, BIS(PYRIDINE-2-CARBOXAMIDONETROPSIN)(CH2)3-6
Record Number:CaltechAUTHORS:CHOpnas95
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:CHOpnas95
Alternative URL:http://www.pnas.org/cgi/content/abstract/92/22/10389
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:888
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:01 Nov 2005
Last Modified:26 Dec 2012 08:41

Repository Staff Only: item control page