Cho, Junhyeong and Parks, Michelle E. and Dervan, Peter B. (1995) Cyclic polyamides for recognition in the minor groove of DNA. Proceedings of the National Academy of Sciences of the United States of America, 92 (22). pp. 10389-10392. ISSN 0027-8424 http://resolver.caltech.edu/CaltechAUTHORS:CHOpnas95
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Small molecules that specifically bind with high affinity to any designated DNA sequence in the human genome would be useful tools in molecular biology and potentially in human medicine. Simple rules have been developed to rationally alter the sequence specificity of minor groove-binding polyamides containing N-methylimidazole and N-methylpyrrole amino acids. Crescent-shaped polyamides bind as antiparallel dimers with each polyamide making specific contacts with each strand on the floor of the minor groove. Cyclic polyamides have now been synthesized that bind designated DNA sequences at subnanomolar concentrations.
|Additional Information:||Copyright © 1995 by the National Academy of Sciences. Contributed by Peter B. Dervan, July 21, 1995. We thank the Mass Spectrometry Laboratory (University of California, Riverside) for the mass spectral analysis. We are grateful to the National Institutes of Health (Grant 27681) for research support and a National Institutes of Health Research Service Award to M.E.P.|
|Subject Keywords:||SEQUENCE-SPECIFIC RECOGNITION, COVALENT PEPTIDE DIMERS, FOOTPRINT TITRATION, 2-DIMENSIONAL NMR, DISTAMYCIN-A, MOTIF, BINDING, COMPLEX, BIS(PYRIDINE-2-CARBOXAMIDONETROPSIN)(CH2)3-6|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Tony Diaz|
|Deposited On:||01 Nov 2005|
|Last Modified:||26 Dec 2012 08:41|
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