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Isolation of metaphase chromosomes from HeLa cells

Huberman, Joel A. and Attardi, Giuseppe (1966) Isolation of metaphase chromosomes from HeLa cells. Journal of Cell Biology, 31 (1). pp. 95-105. ISSN 0021-9525.

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The authors have developed a method for large-scale isolation of metaphase chromosomes from HeLa cells. The distinguishing feature of this method is the use of a pH sufficiently low (about 3) to stabilize the chromosomes against mechanical damage. Many milligrams of fairly pure, morphologically intact chromosomes can be isolated in 8 hr or less of total working time. The isolated chromosomes contain about 2.0 mg of acid-soluble protein, 2.7 mg of acid-insoluble protein and 0.66 mg of RNA for each milligram of DNA. The RNA bound to the isolated chromosomes consists mainly of ribosomal RNA, but there is also a significant amount of 45S RNA.

Item Type:Article
Additional Information:Copyright © 1966 by Rockefeller University Press. Received for publication 4 April 1966. This work was supported by United States Public Health Service grants GM-11726 and 5-FI-GM-21,622. The authors gratefully acknowledge the help of Mr. John Elberfeld in part of this work and the valuable technical assistance of Mrs. Benneta Keeley and Mrs. LaVerne Wenzel.
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ID Code:8903
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Deposited On:25 Sep 2007
Last Modified:26 Dec 2012 09:43

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