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In vitro transformation of lymphoid cells by Abelson murine leukemia virus

Rosenberg, Naomi and Baltimore, David and Scher, Charles D. (1975) In vitro transformation of lymphoid cells by Abelson murine leukemia virus. Proceedings of the National Academy of Sciences of the United States of America, 72 (5). pp. 1932-1936. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:ROSpnas75

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Abstract

Cell cultures prepared from fetal murine liver were infected by Abelson murine leukemia virus. After about 2 weeks, proliferating cells of lymphoid morphology appeared in some of the cultures. Addition of 2-mercaptoethanol to the initial culture medium greatly enhanced the appearance of the lymphoid cells. Immunoglobulin determinants were evident on the cells in some cultures. Continuous passage of the cells in certain cultures was possible and the passaged cells could form tumors after animal inoculation. Because Abelson murine leukemia virus is able to induce in vitro malignant transformation of lymphoid cells, it probably causes leukemia by directly affecting cellular growth control.


Item Type:Article
Additional Information:© 1975 by the National Academy of Sciences. Contributed by David Baltimore, March 7, 1975. We thank Dr. R. Auerbach for helpful discussions, Dr. E. Schneeberger for preparing the electron micrographs and Dr. R. Parkman for performing the cytotoxicity assays. J. Fontaine and C. Bastone provided excellent technical assistance. This work was supported by Grants CA-13472, CA-05616, and CA-14051 from the National Institutes of Health and a contract from the Virus Cancer Program of the National Cancer Institute. C.D.S. was aided by a Cancer Research Scholar Award from the American Cancer Society, Massachusetts Division, Inc. D.B. is an American Cancer Society Research Professor.
Subject Keywords:murine fetal liver; B-lynmphocytes; surface immunoglobulin; mercaptoethanol; murine lymphosarcoma
Record Number:CaltechAUTHORS:ROSpnas75
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:ROSpnas75
Alternative URL:http://dx.doi.org/10.1073/pnas.72.5.1932
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8917
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:01 Oct 2007
Last Modified:14 Nov 2014 19:20

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