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Expression of Drosophila Shaker potassium channels in mammalian cells infected with recombinant vaccinia virus

Leonard, R. J. and Karschin, A. and Jayashree-Aiyar, S. and Davidson, N. and Tanouye, M. A. and Thomas, L. and Thomas, G. and Lester, H. A. (1989) Expression of Drosophila Shaker potassium channels in mammalian cells infected with recombinant vaccinia virus. Proceedings of the National Academy of Sciences of the United States of America, 86 (19). pp. 7629-7633. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:LEOpnas89

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Abstract

A recombinant vaccinia virus containing a Drosophila potassium channel (Shaker H4) cDNA was constructed by homologous recombination between wild-type vaccinia virus DNA and a transfer plasmid. The new virus was used to infect four types of mammalian cells in culture. Electrophysiological recording 24-72 hr after infection revealed the expression of voltage-gated transient potassium channels in all four cell types. The properties of the induced currents were identical to those previously observed following injection of the Shaker H4 transcript into oocytes. Vaccinia promises to be an effective vehicle for the heterologous expression of transmembrane ion channels in a variety of cell types.


Item Type:Article
Additional Information:© 1989 by the National Academy of Sciences. Contributed by N. Davidson, July 14, 1989. We thank Barbara Thorne for expert assistance in the construction of the recombinant virus and J.H. Strauss and R.W. Aldrich for advice. Charybdotoxin was a generous gift of Dr. M.L. Garcia (Merck Sharp & Dohme Research Laboratories). This work was supported by National Institutes of Health Grants GM10991, GM29836, and DK37274, by the Cystic Fibrosis Foundation, and by fellowships from the Max Kade Foundation (A.K.) and the National Institutes of Health (R.J.L.). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:heterologous expression; rat basophilic leukemia cells; rat pheochromocytoma cells
Record Number:CaltechAUTHORS:LEOpnas89
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:LEOpnas89
Alternative URL:http://www.pnas.org/cgi/content/abstract/86/19/7629
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8918
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:01 Oct 2007
Last Modified:14 Nov 2014 19:20

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