Goulian, Mehran and Kornberg, Arthur and Sinsheimer, Robert L. (1967) Enzymatic synthesis of DNA, XXIV. Synthesis of infectious phage phiX174 DNA. Proceedings of the National Academy of Sciences of the United States of America, 58 (6). pp. 2321-2328. ISSN 0027-8424 http://resolver.caltech.edu/CaltechAUTHORS:GOUpnas67
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Abstract
Past attempts at in vitro replication of transforming factor present in DNA have given negative or inconclusive results.(1-3) Rigid proof was lacking that template material had been excluded from the synthetic product. Even if a rigorous demonstration of net synthesis of transforming factor for a given genetic marker were forthcoming, it would still prove only that some relatively short sequence of nucleotides, sufficient for replacement of the mutant locus, had been synthesized. If enzymatic synthesis of infectious bacteriophage DNA were achieved, it would be made clear at once that relatively few, if any, mistakes had been made in replicating a DNA sequence of several thousand nucleotides.
| Item Type: | Article |
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| Additional Information: | © 1967 by the National Academy of Sciences. Communicated September 25, 1967. We gratefully acknowledge the expert assistance of Mrs. Gloria Davis of the Division of Biology at the California Institute of Technology in performing the spheroplast assays for infectivity. This research was supported by grants from the National Institutes of Health and the National Science Foundation. [M.G. was an] USPHS special fellow. |
| Record Number: | CaltechAUTHORS:GOUpnas67 |
| Persistent URL: | http://resolver.caltech.edu/CaltechAUTHORS:GOUpnas67 |
| Alternative URL: | http://www.pnas.org/cgi/reprint/58/6/2321 |
| Usage Policy: | You are granted permission for individual, educational, research and non-commercial reproduction, distribution, display and performance of this work in any format. |
| Metadata Review: | All Records > Caltech Library Services |
| ID Code: | 8973 |
| Collection: | CaltechAUTHORS |
| Deposited By: | Tony Diaz |
| Deposited On: | 11 Oct 2007 |
| Last Modified: | 04 Jul 2008 21:05 |
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