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A method for selection of biochemical mutants of Neurospora

Lein, Joseph and Mitchell, Herschel K. and Houlahan, Mary B. (1948) A method for selection of biochemical mutants of Neurospora. Proceedings of the National Academy of Sciences of the United States of America, 34 (9). pp. 435-442. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:LEIpnas48

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Abstract

Numerous investigations in recent-years have firmly established the value of mutants of the mold Neurospora as tools for research in biochemistry and genetics.(1,5,6) Although various means have been used for inducing mutations,(4,5,6) the technique of isolation as described by Beadle and Tatum(4) has been used by most investigators. This method involves isolation of single ascospores, one from each perithecium, derived from a cross of which one parent was treated. By this method at least 97 per cent of the cultures obtained from the single spore isolations are unchanged wild type strains. In order to obtain specific mutants or a diversity of mutants, it is therefore necessary to isolate a very large number of single ascospores.


Item Type:Article
Additional Information:© 1948 by the National Academy of Sciences. Communicated by G. W. Beadle, June 15, 1948. Work supported in part by a grant from the Rockefeller Foundation and in part through a contract with the Office of Naval Research of the United States Navy Department. [J.L. was a] Merck Research Fellow of the National Research Council.
Record Number:CaltechAUTHORS:LEIpnas48
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:LEIpnas48
Alternative URL:http://www.pnas.org/cgi/reprint/34/9/435
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9008
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:20 Oct 2007
Last Modified:26 Dec 2012 09:44

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