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Structure of a nicked DNA-protein complex isolated from simian virus 40: Covalent attachment of the protein to DNA and nick specificity

Kasamatsu, Harumi and Wu, Madeline (1976) Structure of a nicked DNA-protein complex isolated from simian virus 40: Covalent attachment of the protein to DNA and nick specificity. Proceedings of the National Academy of Sciences of the United States of America, 73 (6). pp. 1945-1949. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:KASpnas76

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Abstract

A portion of the nicked circular DNA isolated from purified simian virus 40 contains a protein-DNA complex in which protein(s) is covalently attached to the end of a DNA single strand. (Nicked DNA is double-stranded DNA that contains at least one single-strand scission.) The protein was visualized by electron microscopy and labeled in vitro with 125I. The bond between the protein and the DNA is stable in alkali, 4 M guanidine· hydrochloride, 3.86 M hydroxylamine (pH 4.23), and in 98% formamide. Most of the molecules in the nicked circular DNA fraction contained one nick. The nick occurs on either of the two complementary strands; the specific nick sites on the two strands are staggered, but lie within a few hundred nucleotides of each other.


Item Type:Article
Additional Information:Copyright © 1976 by the National Academy of Sciences. Communicated by Jerome Vinograd, April 5, 1976. We are indebted to Profs. J. Vinograd and N. Davidson, in whose laboratories this work was conducted, for advice, encouragement, and criticism. This research has been supported by National Institutes of Health Grants CA08014, GM20927, and GM10991.
Subject Keywords:protein-DNA bonding; electron microscopy; iodination of proteins
Record Number:CaltechAUTHORS:KASpnas76
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:KASpnas76
Alternative URL:http://www.pnas.org/cgi/content/abstract/73/6/1945
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9050
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:23 Oct 2007
Last Modified:26 Dec 2012 09:45

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