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The role of Tat in the human immunodeficiency virus life cycle indicates a primary effect on transcriptional elongation

Feinberg, Mark B. and Baltimore, David and Frankel, Alan D. (1991) The role of Tat in the human immunodeficiency virus life cycle indicates a primary effect on transcriptional elongation. Proceedings of the National Academy of Sciences of the United States of America, 88 (9). pp. 4045-4049. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:FEIpnas91

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Abstract

The mechanism of Tat transactivation was studied by treating cell lines containing Tat-defective viruses with purified Tat protein. These cell lines constitutively produce very low levels of virus in the absence of Tat, as measured by p24 antigen levels. Virus production can be increased >30,000-fold by adding exogenous Tat. Tat addition increases mRNA levels early in the viral life cycle, and Tat is required for Rev function to become evident. There is no evidence for a translational effect of Tat. Nuclear run-on experiments show that the increase in mRNA levels is due to an increased efficiency of elongation of nascent transcripts. These results suggest that Tat may be a gene-specific elongation factor.


Item Type:Article
Additional Information:© 1991 by the National Academy of Sciences. Contributed by David Baltimore, January 28, 1991. We thank A. Sachs, D. Black, M. Laspia, R. Andino, and M. Muesing for helpful discussions; A. Aldovini for help with preliminary experiments; and D. Vollrath and S. Buratowski for comments on the manuscript. This work was supported by the Lucille P. Markey Charitable Trust and by National Institutes of Health Grant AI29135 (A.D.F.), and by National Institutes of Health Grants AI26463 and GM39458 (D.B.). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:VIRAL TRANSACTIVATOR; TRANSCRIPTIONAL ELONGATION; ELONGATION FACTOR
Record Number:CaltechAUTHORS:FEIpnas91
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:FEIpnas91
Alternative URL:http://dx.doi.org/10.1073/pnas.88.9.4045
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9210
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:27 Nov 2007
Last Modified:14 Nov 2014 19:20

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