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Structure of 3,4-Dihydroxy-2-butanone 4-Phosphate Synthase from Methanococcus jannaschii in Complex with Divalent Metal Ions and the Substrate Ribulose 5-Phosphate: implications for the catalytic mechanism

Steinbacher, Stefan and Schiffmann, Susanne and Richter, Gerald and Huber, Robert and Bacher, Adelbert and Fischer, Markus (2003) Structure of 3,4-Dihydroxy-2-butanone 4-Phosphate Synthase from Methanococcus jannaschii in Complex with Divalent Metal Ions and the Substrate Ribulose 5-Phosphate: implications for the catalytic mechanism. Journal of Biological Chemistry, 278 (43). pp. 42256-42265. ISSN 0021-9258. http://resolver.caltech.edu/CaltechAUTHORS:STEjbc03a

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Abstract

Skeletal rearrangements of carbohydrates are crucial for many biosynthetic pathways. In riboflavin biosynthesis ribulose 5-phosphate is converted into 3,4-dihydroxy-2-butanone 4-phosphate while its C4 atom is released as formate in a sequence of metal-dependent reactions. Here, we present the crystal structure of Methanococcus jannaschii 3,4-dihydroxy-2-butanone 4-phosphate synthase in complex with the substrate ribulose 5-phosphate at a dimetal center presumably consisting of non-catalytic zinc and calcium ions at 1.7-Å resolution. The carbonyl group (O2) and two out of three free hydroxyl groups (OH3 and OH4) of the substrate are metal-coordinated. We correlate previous mutational studies on this enzyme with the present structural results. Residues of the first coordination sphere involved in metal binding are indispensable for catalytic activity. Only Glu-185 of the second coordination sphere cannot be replaced without complete loss of activity. It contacts the C3 hydrogen atom directly and probably initiates enediol formation in concert with both metal ions to start the reaction sequence. Mechanistic similarities to Rubisco acting on the similar substrate ribulose 1,5-diphosphate in carbon dioxide fixation as well as other carbohydrate (reducto-) isomerases are discussed.


Item Type:Article
Additional Information:Copyright © 2003 by the American Society for Biochemistry and Molecular Biology. Received for publication, July 8, 2003 , and in revised form, August 5, 2003. Originally published In Press as doi:10.1074/jbc.M307301200 on August 6, 2003. We thank Richard Feicht for help with the preparation of the protein. The atomic coordinates and structure factors (code 1PVW and 1PVY) have been deposited in the Protein Data Bank, Research Collaboratory for Structural Bioinformatics, Rutgers University, New Brunswick, NJ (http://www.rcsb.org/). This work was supported by the Deutsche Forschungsgemeinschaft and the Fonds der Chemischen Industrie. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Record Number:CaltechAUTHORS:STEjbc03a
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:STEjbc03a
Alternative URL:http://dx.doi.org/10.1074/jbc.M307301200
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ID Code:9228
Collection:CaltechAUTHORS
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Deposited On:28 Nov 2007
Last Modified:26 Dec 2012 09:46

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