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Characterization and cloning of fasciclin I and fasciclin II glycoproteins in the grasshopper

Snow, Peter M. and Zinn, Kai and Harrelson, Allan L. and McAllister, Linda and Schilling, Jim and Bastiani, Michael J. and Makk, George and Goodman, Corey S. (1988) Characterization and cloning of fasciclin I and fasciclin II glycoproteins in the grasshopper. Proceedings of the National Academy of Sciences of the United States of America, 85 (14). pp. 5291-5295. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:SNOpnas88

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Abstract

Monoclonal antibodies were previously used to identify two glycoproteins, called fasciclin I and II (70 and 95 kDa, respectively), which are expressed on different subsets of axon fascicles in the grasshopper (Schistocerca americana) embryo. Here the monoclonal antibodies were used to purify these two membrane-associated glycoproteins for further characterization. Fasciclin II appears to be an integral membrane protein, where fasciclin I is an extrinsic membrane protein. The amino acid sequences of the amino terminus and fragments of both proteins were determined. Using synthetic oligonucleotide probes and antibody screening, we isolated genomic and cDNA clones. Partial DNA sequences of these clones indicate that they encode fasciclins I and II.


Item Type:Article
Additional Information:Copyright © 1988 by the National Academy of Sciences. Communicated by Gerald M. Rubin, March 9, 1988. We thank Zaida Traquina and Violette Paragas for technical assistance. We also thank Hans Acha-Orbea and Ed Fritsch for helpful discussions. This work was supported by American Cancer Society Postdoctoral Fellowships to P.M.S. and A.L.H., a Helen Hay Whitney Postdoctoral Fellowship to K.Z., a National Institutes of Health Medical Scientist Training Program Traineeship to L.M., National Institute of Mental Health Grant MH 23861-13 to G.M., and grants and awards from the National Institutes of Health, McKnight Foundation, and March of Dimes-Birth Defects Foundation to C.S.G. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:membrane proteins, immunoaffinity purification, neuronal recognition, growth cone guidance
Record Number:CaltechAUTHORS:SNOpnas88
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:SNOpnas88
Alternative URL:http://www.pnas.org/cgi/content/abstract/85/14/5291
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:937
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:15 Nov 2005
Last Modified:26 Dec 2012 08:42

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