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Nitrogen distribution by globin

Hunter, Andrew and Borsook, Henry (1923) Nitrogen distribution by globin. Journal of Biological Chemistry, 57 (2). pp. 507-514. ISSN 0021-9258. http://resolver.caltech.edu/CaltechAUTHORS:HUNjbc23

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Abstract

This and other experiences with the tryptophane method of Fürth and Nobel led us to doubt seriously the reliability of quantitative data obtained by its application. When, therefore, just as we completed our work with it, Folin and Looney (6) described another and apparently better method of determination, a method based upon a different color reaction and capable moreover of convenient combination with a quantitative procedure for tyrosine, it seemed to us worth while to review the problem again. With the aid of this newer method we have now determined the tryptophane and tyrosine content of two series of globin preparations, and have, we believe, settled fairly decisively the proportion of these amino-acids yielded by the pure protein. We have also taken occasion to determine by the method of Van Slyke the general distribution of nitrogen in the globin molecule.


Item Type:Article
Additional Information:Copyright © 1923 American Society of Biological Chemists. (Received for publication, July 2, 1923.)
Record Number:CaltechAUTHORS:HUNjbc23
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:HUNjbc23
Alternative URL:http://www.jbc.org/content/vol57/issue2/index.shtml
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9373
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:16 Dec 2007
Last Modified:26 Dec 2012 09:48

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