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Inhibition of translation by poliovirus: Inactivation of a specific initiation factor

Rose, John K. and Trachsel, Hans and Leong, Kahan and Baltimore, David (1978) Inhibition of translation by poliovirus: Inactivation of a specific initiation factor. Proceedings of the National Academy of Sciences of the United States of America, 75 (6). pp. 2732-2736. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:ROSpnas78

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Abstract

Translation of vesicular stomatitis virus (VSV) mRNA, like host mRNA translation, is inhibited in cells infected with poliovirus. To study the mechanism of poliovirus-induced inhibition of protein synthesis, we prepared extracts from poliovirus-infected and uninfected HeLa cells. Poliovirus mRNA was translated in lysates from both infected and uninfected cells, while VSV mRNA was translated only in the lysate from uninfected cells. Addition of purified translation initiation factors to the extract from infected cells showed that one factor, eIF-4B, could restore VSV mRNA translation in the infected lysate, but did not increase poliovirus mRNA translation. Further experiments involving translation of VSV mRNA in mixed extracts from poliovirus-infected and uninfected cells showed (i) that there was not an excess of an inhibitor of VSV mRNA translation in the infected lysate, but (ii) that an activity that caused a slow inactivation of eIF-4B was present in the infected lysate. Inactivation of eIF-4B appears to be the mechanism by which poliovirus infection causes a selective inhibition of translation.


Item Type:Article
Additional Information:© 1978 by the National Academy of Sciences. Contributed by David Baltimore, April 7, 1978. We are grateful to Dr. Irving London for providing the support and facilities for the initiation factor purification and to Marilyn Smith for preparation of the manuscript. This work was supported by Grant AI14566 to J.K.R., Grant AI08388 to D.B., Program Project Grant CA12174, Core Grant CA14051 to Dr. S. Luria, and National Institutes of Health Grant AM16272 to Dr. Irving London. D.B. is an American Cancer Society Research Professor. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:translational control; eukaryotic initiation factor-4B; in vitro translation; vesicular stomatitis virus
Record Number:CaltechAUTHORS:ROSpnas78
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:ROSpnas78
Alternative URL:http://dx.doi.org/10.1073/pnas.75.6.2732
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9449
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:07 Jan 2008
Last Modified:26 Dec 2012 09:48

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