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Equilibrium sedimentation of macromolecules in density gradients

Meselson, Matthew and Stahl, Franklin W. and Vinograd, Jerome (1957) Equilibrium sedimentation of macromolecules in density gradients. Proceedings of the National Academy of Sciences of the United States of America, 43 (7). pp. 581-588. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:MESpnas57

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Abstract

This communication presents a new method for the study of the molecular weight and partial specific volume of macromolecules, with some illustrations based on results with deoxyribonucleic acid (DNA) and several viruses. The method involves observation of the equilibrium distribution of macromolecular material in a density gradient itself at equilibrium. The density gradient is established by the sedimentation of a low-molecular-weight solute in a solution subject to a constant centrifugal field.


Item Type:Article
Additional Information:© 1957 by the National Academy of Sciences. Communicated by Linus Pauling, May 27, 1957. We gratefully acknowledge the technical assistance of Mr. Richard Holmquist, Mrs. Ilga Liclausis, and Mrs. Janet Morris. Mr. Darwin Smith rendered valuable assistance with the photometry. We are indebted to Dr. John Lamperti and to Mr. Charles Steinberg for many valuable discussions. Aided by grants from the National Institutes of Health and the National Foundation for Infantile Paralysis. [M.M. was a] Predoctoral Fellow of the National Science Foundation. [F.W.S. was a]Fellow in the Medical Sciences of the National Research Council. Gates and Crellin Laboratories of Chemistry, Contribution No. 2205.
Record Number:CaltechAUTHORS:MESpnas57
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:MESpnas57
Alternative URL:http://www.pnas.org/cgi/reprint/43/7/581
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9477
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:23 Jan 2008
Last Modified:26 Dec 2012 09:49

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