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Deletion mutants of bacteriophage phiX174

Zuccarelli, Anthony J. and Benbow, Robert M. and Sinsheimer, Robert L. (1972) Deletion mutants of bacteriophage phiX174. Proceedings of the National Academy of Sciences of the United States of America, 69 (7). pp. 1905-1910. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:ZUCpnas72

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Abstract

Mutants of bacteriophage phiX174 have been isolated that are less dense than wild-type phiX particles in CsCl. When mutant viral (+) strand DNA and wild-type complementary (-) strand DNA are hybridized, the resulting duplex molecules have single-stranded loops characteristic of wild-type-deletion heteroduplexes. The mutant bacteriophages fail to complement phiX amber mutants in cistron E. We conclude that the mutant viruses have deleted approximately 7% of the phiX genome in the region of cistron E.


Item Type:Article
Additional Information:© 1972 by the National Academy of Sciences. Contributed by Robert L. Sinsheimer, April 24, 1972. We are deeply indebted to J.S. Kim, P.A. Sharp, and N. Davidson for their patient instruction in the preparation of samples for electron microscopy and for their suggestions in the writing of this manuscript. This research was supported by USPHS Grant GM-13554, USPHS Training Grant GM-00086, and by a National Science Foundation Graduate Fellowship.
Subject Keywords:electron microscopy; DNA heteroduplex; density gradient centrifugation; polyacrylamide gel electrophoresis
Record Number:CaltechAUTHORS:ZUCpnas72
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:ZUCpnas72
Alternative URL:http://www.pnas.org/cgi/content/abstract/69/7/1905
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9479
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:23 Jan 2008
Last Modified:14 Nov 2014 19:20

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