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Nonrepetitive DNA sequence representation in sea urchin embryo messenger RNA

Goldberg, Robert B. and Galau, Glenn A. and Britten, Roy J. and Davidson, Eric H. (1973) Nonrepetitive DNA sequence representation in sea urchin embryo messenger RNA. Proceedings of the National Academy of Sciences of the United States of America, 70 (12, Pt). pp. 3516-3520. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:GOLpnas73

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Abstract

Messenger RNA was prepared from developing sea urchin gastrulae by puromycin release from polyribosomes. Approximately 60% of the total mRNA radioactivity of the postnuclear supernatant was recovered and shown to be free of any other labeled RNA species such as ribosomal and nuclear RNA. The mRNA was examined by hybridization to DNA present in great excess. The mRNA hybridizes almost exclusively with nonrepetitive DNA. Almost all of the messenger RNA molecules of sea urchin gastrulae therefore consist of transcripts from nonrepetitive sequences. It appears that the structural genes expressed at this stage are typically not repeated in the genome and the mRNA does not include recognizable repetitive sequence.


Item Type:Article
Additional Information:© 1973 by the National Academy of Sciences. Contributed by Roy J. Britten, July 13, 1973. This research was supported by National Science Foundation Grant GB-33441X and by a grant from the National Institutes of Health (HD05753). R.B.G. was the recipient of an NIH Postdoctoral Fellowship GM51391. G.A.G. is a predoctoral fellow on USPH Training Grant GM 00086.
Subject Keywords:3H-labeled gastrula mRNA; hybridization; hydroxyapatite
Record Number:CaltechAUTHORS:GOLpnas73
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:GOLpnas73
Alternative URL:http://www.pnas.org/cgi/content/abstract/70/12/3516
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9565
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:05 Feb 2008
Last Modified:26 Dec 2012 09:50

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