Goldberg, Robert B. and Galau, Glenn A. and Britten, Roy J. and Davidson, Eric H. (1973) Nonrepetitive DNA sequence representation in sea urchin embryo messenger RNA. Proceedings of the National Academy of Sciences of the United States of America, 70 (12, Pt). pp. 3516-3520. ISSN 0027-8424 http://resolver.caltech.edu/CaltechAUTHORS:GOLpnas73
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Messenger RNA was prepared from developing sea urchin gastrulae by puromycin release from polyribosomes. Approximately 60% of the total mRNA radioactivity of the postnuclear supernatant was recovered and shown to be free of any other labeled RNA species such as ribosomal and nuclear RNA. The mRNA was examined by hybridization to DNA present in great excess. The mRNA hybridizes almost exclusively with nonrepetitive DNA. Almost all of the messenger RNA molecules of sea urchin gastrulae therefore consist of transcripts from nonrepetitive sequences. It appears that the structural genes expressed at this stage are typically not repeated in the genome and the mRNA does not include recognizable repetitive sequence.
|Additional Information:||© 1973 by the National Academy of Sciences. Contributed by Roy J. Britten, July 13, 1973. This research was supported by National Science Foundation Grant GB-33441X and by a grant from the National Institutes of Health (HD05753). R.B.G. was the recipient of an NIH Postdoctoral Fellowship GM51391. G.A.G. is a predoctoral fellow on USPH Training Grant GM 00086.|
|Subject Keywords:||3H-labeled gastrula mRNA; hybridization; hydroxyapatite|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Tony Diaz|
|Deposited On:||05 Feb 2008|
|Last Modified:||26 Dec 2012 09:50|
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