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Membrane penetration of Sendai virus glycoproteins during the early stages of fusion with liposomes as determined by hydrophobic photoaffinity labeling

Novick, Steven L. and Hoekstra, Dick (1988) Membrane penetration of Sendai virus glycoproteins during the early stages of fusion with liposomes as determined by hydrophobic photoaffinity labeling. Proceedings of the National Academy of Sciences of the United States of America, 85 (20). pp. 7433-7437. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:NOVpnas88

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Abstract

The hydrophobic photoaffinity label 3-(trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine was used to label Sendai virus proteins during fusion with cardiolipin and phosphatidylserine liposomes. Preferential labeling of the viral fusion protein during the initial stages of fusion demonstrated that this protein interacts with the hydrophobic core of the target membrane as an initiating event of virus-liposome fusion. Labeling showed time, temperature, and pH dependence consistent with earlier fluorescent measurements of fusion kinetics. The present method provides conclusive evidence supporting the hypothesis that hydrophobic interaction of the fusion protein with the target bilayer is an initial event in the fusion mechanism of viral membranes.


Item Type:Article
Additional Information:© 1988 by the National Academy of Sciences. Communicated by John D. Baldeschwieler, May 19, 1988. We wish to thank Karin Klappe for expert technical assistance and to acknowledge the helpful suggestions of Dr. Peter Zahler (Laboratory of Biochemistry, University of Bern, Bern, Switzerland) during the early stages of this work and Drs. John D. Baldeschwieler and Robert A. Copeland for critical readings of the manuscript. This work was supported by U.S. Army Research Office Grant DAAG29-83-K-0128, National Institutes of Health Biomedical Research Support Grant RR07003 administered by the Division of Research Resources, and grants from Monsanto and Amersham. S.L.N. is the recipient of a National Science Foundation Predoctoral Research Fellowship. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:membrane fusion; virus infection
Record Number:CaltechAUTHORS:NOVpnas88
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:NOVpnas88
Alternative URL:http://www.pnas.org/cgi/content/abstract/85/20/7433
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9608
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:18 Feb 2008
Last Modified:26 Dec 2012 09:50

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