Spector, Deborah H. and Baltimore, David (1974) Requirement of 3'-terminal poly(adenylic acid) for the infectivity of poliovirus RNA. Proceedings of the National Academy of Sciences of the United States of America, 71 (8). pp. 2983-2987. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:SPEpnas74
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Ribonuclease H (EC 220.127.116.11) has been used to remove selectively much of the 3'-terminal poly(adenylic acid) [poly(A)] from poliovirus RNA by treating the RNA with the enzyme in the presence of poly(dT). Over 80% of the poly(A) could be removed and the residuum was found as oligo(A) stretches attached to many or all of the viral RNA molecules. Reduction of the size of the poly(A) markedly decreased the specific infectivity of poliovirus RNA, indicating that poly(A) is necessary to the infectivity of the RNA. The virions in plaques deriving from infection with treated RNA have a normal amount and size of poly(A), indicating that mechanisms exist in infected cells to regenerate normal length poly(A) from truncated poly(A).
|Additional Information:||© 1974 by the National Academy of Sciences. Contributed by David Baltimore, May 16, 1974. The work was supported by Grant AI-08388 from the National Institutes of Health. D.H.S. is a pre-doctoral fellow of the National Science Foundation. D.B. is an American Cancer Society Research professor.|
|Subject Keywords:||ribonuclease H; removal of poly(A)|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Tony Diaz|
|Deposited On:||26 Mar 2008|
|Last Modified:||26 Dec 2012 09:53|
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