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SSB-1 of the yeast Saccharomyces cerevisiae is a nucleolar-specific, silver-binding protein that is associated with the snR10 and snR11 small nuclear RNAs

Clark, Michael W. and Yip, Man Lun R. and Campbell, Judith and Abelson, John (1990) SSB-1 of the yeast Saccharomyces cerevisiae is a nucleolar-specific, silver-binding protein that is associated with the snR10 and snR11 small nuclear RNAs. Journal of Cell Biology, 111 (5). pp. 1741-1751. ISSN 0021-9525. http://resolver.caltech.edu/CaltechAUTHORS:CLAjcb90

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Abstract

SSB-1, the yeast single-strand RNA-binding protein, is demonstrated to be a yeast nucleolar-specific, silver-binding protein. In double-label immunofluorescence microscopy experiments antibodies to two other nucleolar proteins, RNA Pol I 190-kD and fibrillarin, were used to reveal the site of rRNA transcription; i.e., the fibrillar region of the nucleolus. SSB-1 colocalized with fibrillarin in a double-label immunofluorescence mapping experiment to the yeast nucleolus. SSB-1 is located, though, over a wider region of the nucleolus than the transcription site marker. Immunoprecipitations of yeast cell extracts with the SSB-1 antibody reveal that in 150 mM NaCl SSB-1 is bound to two small nuclear RNAs (snRNAs). These yeast snRNAs are snR10 and snR11, with snR10 being predominant. Since snR10 has been implicated in pre-rRNA processing, the association of SSB-1 and snR10 into a nucleolar snRNP particle indicates SSB-1 involvement in rRNA processing as well. Also, another yeast protein, SSB-36-kD, isolated by single- strand DNA chromatography, is shown to bind silver under the conditions used for nucleolar-specific staining. It is, most likely, another yeast nucleolar protein.


Item Type:Article
Additional Information:Copyright © 1990 by The Rockefeller University Press. Received for publication 27 March 1990 and in revised form 27 July 1990. We thank A. Sentenac at Service de Biochimie, Gif-sur-Yvette, Cedex, France and E. Tan at Scripps Clinic, La Jolla, Ca, for the antisera to RNA Pol I 190-kD protein and fibrillarin (mouse monoclonal), respectively. We thank A. Jong at the University of Southern California, Los Angeles, CA, and M. Cusick at Texas A&M University, College Station, TX, for the purified SSB-1 protein and ssDNA cellulose column fractions. We thank E. Shuster at the University of California, San Francisco, CA for the clones of the yeast snRNAs and E. Jones at Carnegie-Mellon University, Pittsburgh, PA, for yeast strain EJ101. M.W. Clark was supported by the National Institutes of Health (NIH) postdoctoral fellowship 5732 GM 1008-02. M.L.R. Yip was supported by Howard Hughes Institute Doctoral Fellowship in Biological Sciences. J. Campbell was supported by NIH grant GM 25588. J. Abelson was supported by NIH grant GM 3267.
Record Number:CaltechAUTHORS:CLAjcb90
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:CLAjcb90
Alternative URL:http://www.jcb.org/cgi/content/abstract/111/5/1741
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Deposited On:26 Mar 2008
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