Cantor, Charles R. (1976) A possible explanation for the nuclease limit digestion pattern of chromatin. Proceedings of the National Academy of Sciences of the United States of America, 73 (10). pp. 3391-3393. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:CANpnas76
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The general pattern of DNA fragments in the limit digest of nuclease-treated chromatin could arise from a single, unique nuclease-susceptible site per nucleosome. If DNA binds to the histone core of the nucleosome along a circularly re-entrant path, the location of the DNA entrance and exit can occur at any of a number of distinct sites. This very specific type of heterogeneity together with the natural 10-fold periodicity of DNA B can account for the observed digestion pattern. Such a general picture of the nucleosome structure could also easily explain how nucleosomes might move along the DNA. This type of structure should be easy to distinguish experimentally from more conventional explanations of the origin of the limit digest pattern of chromatin.
|Additional Information:||© 1976 by the National Academy of Sciences. Communicated by Jerome Vinograd, July 26, 1976. I am grateful to Gary Felsenfeld for stimulating my interest in the problem and to Robert Stroud and David Pulleyblank for several extremely helpful discussions. This work was supported by the Fairchild Scholar Program of the California Institute of Technology. This is Contribution no. 5375 from the Division of Chemistry and Chemical Engineering.|
|Subject Keywords:||nucleosome structure; re-entrant binding; DNA movement|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Tony Diaz|
|Deposited On:||26 Mar 2008|
|Last Modified:||26 Dec 2012 09:54|
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