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An improved whole life cycle culture protocol for the hydrozoan genetic model Clytia hemisphaerica

Lechable, Marion and Jan, Alexandre and Duchene, Axel and Uveira, Julie and Weissbourd, Brandon and Gissat, Loann and Collet, Sophie and Gilletta, Laurent and Chevalier, Sandra and Leclère, Lucas and Peron, Sophie and Barreau, Carine and Lasbleiz, Régis and Houliston, Evelyn and Momose, Tsuyoshi (2020) An improved whole life cycle culture protocol for the hydrozoan genetic model Clytia hemisphaerica. Biology Open, 9 (11). Art. No. bio051268. ISSN 2046-6390. PMCID PMC7657476. https://resolver.caltech.edu/CaltechAUTHORS:20191125-154148136

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Abstract

The jellyfish species Clytia hemisphaerica (Cnidaria, Hydrozoa) has emerged as a new experimental model animal in the last decade. Favorable characteristics include a fully transparent body suitable for microscopy, daily gamete production and a relatively short life cycle. Furthermore, whole genome sequence assembly and efficient gene editing techniques using CRISPR/Cas9 have opened new possibilities for genetic studies. The quasi-immortal vegetatively-growing polyp colony stage provides a practical means to maintain mutant strains. In the context of developing Clytia as a genetic model, we report here an improved whole life cycle culture method including an aquarium tank system designed for culture of the tiny jellyfish form. We have compared different feeding regimes using Artemia larvae as food and demonstrate that the stage-dependent feeding control is the key for rapid and reliable medusa and polyp rearing. Metamorphosis of the planula larvae into a polyp colony can be induced efficiently using a new synthetic peptide. The optimized procedures detailed here make it practical to generate genetically modified Clytia strains and to maintain their whole life cycle in the laboratory.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1242/bio.051268DOIArticle
https://doi.org/10.1101/852632DOIDiscussion Paper
https://www.embrc-france.fr/frRelated ItemStrains
http://marimba.obs-vlfr.frRelated ItemMarimba Database
https://bio.biologists.org/content/9/11/bio051268.supplementalPublisherSupplementary information
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7657476PubMed CentralArticle
ORCID:
AuthorORCID
Weissbourd, Brandon0000-0001-5422-3873
Momose, Tsuyoshi0000-0002-3806-3408
Additional Information:© 2020 Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Received February 11, 2020; Accepted September 7, 2020; Published 5 November 2020. We thank Maciej Mańko for making new A-series wild-type strains during his stay in Villefranche-sur-Mer. Clytia hemisphaerica wild-type strains used in this work are available from CRBM (https://www.embrc-france.fr/fr). Funding: Agence Nationale de la Recherche (ANR), ANR-17-CE13-0016 (i-MMEJ); European Union, Horizon 2020 Research and Innovation programme No 730984, ASSEMBLE Plus project; BW is a Howards Hughes Medical Institute Fellow of the Life Sciences Research Foundation; the CRBM is supported by EMBRC-France, whose French state funds are managed by the ANR within the Investments of the Future program under reference ANR-10-INBS-02. Clytia strain sequencing and database development received funding the European Union, Horizon 2020 Research and Innovation programme under grant agreement 654248, CORBEL project (Available in Marimba database http://marimba.obs-vlfr.fr). Author contributions: Conceptualization: T.M.; Methodology: M.L., A.J., A.D., J.U., B.W., L. Gissat, S. Collet, S. Chevalier, L.L., S.P., T.M.; Validation: J.U., L. Gilletta; Investigation: M.L., A.J., A.D.; Resources: A.J., L. Gissat, S. Collet, L. Gilletta, S. Chevalier, R.L., E.H., T.M.; Data curation: M.L.; Writing - original draft: T.M.; Writing - review & editing: M.L., T.M.; Visualization: M.L.; Supervision: J.U., B.W., L.L., C.B., R.L., T.M.; Project administration: T.M.; Funding acquisition: R.L., T.M., B.W., E.H. The authors declare no competing or financial interests.
Funders:
Funding AgencyGrant Number
Agence Nationale pour la Recherche (ANR)ANR-17-CE13-0016
European Research Council (ERC)730984
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
European Marine Biological Resource Center (EMBRC)UNSPECIFIED
Agence Nationale pour la Recherche (ANR)ANR-10-INBS-02
European Research Council (ERC)654248
Subject Keywords:Animal culture; Cnidarian; Developmental biology; Genetics; Jellyfish
Issue or Number:11
PubMed Central ID:PMC7657476
Record Number:CaltechAUTHORS:20191125-154148136
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20191125-154148136
Official Citation:An improved whole life cycle culture protocol for the hydrozoan genetic model Clytia hemisphaerica. Marion Lechable, Alexandre Jan, Axel Duchene, Julie Uveira, Brandon Weissbourd, Loann Gissat, Sophie Collet, Laurent Gilletta, Sandra Chevalier, Lucas Leclère, Sophie Peron, Carine Barreau, Régis Lasbleiz, Evelyn Houliston, Tsuyoshi Momose. Biology Open 2020 9: bio051268 doi: 10.1242/bio.051268 Published 5 November 2020
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:100049
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:26 Nov 2019 00:05
Last Modified:16 Nov 2020 17:16

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