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Construction of an inducible amyloid expression circuit in Bacillus megaterium: A case study with CsgA and TasA

Larsson, Elin M. and McManus, John B. and Murray, Richard M. (2019) Construction of an inducible amyloid expression circuit in Bacillus megaterium: A case study with CsgA and TasA. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20191202-140340910

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Abstract

Environmental applications of synthetic biology such as water remediation require engineered strains to function robustly in a fluctuating and potentially hostile environment. The construction of synthetic biofilm formation circuits could potentially alleviate this issue by promoting cell survival. Towards this end, we construct a xylose-inducible system for the expression of the functional amyloids CsgA and TasA in the soil bacterium Bacillus megaterium. We find that although both amyloids are expressed, only TasA is successfully exported from the cells. Furthermore, expression of CsgA results in a significant growth penalty for the cells while expression of TasA does not. Finally, we show that TasA expression conveys a small but detectable increase in cells’ adhesion to nickel beads. These results suggest that TasA is a promising candidate for future work on synthetic biofilm formation in B. megaterium.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/858266DOIDiscussion Paper
ORCID:
AuthorORCID
Larsson, Elin M.0000-0003-1341-5937
Murray, Richard M.0000-0002-5785-7481
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license. bioRxiv preprint first posted online Nov. 29, 2019. This research is supported by the Institute for Collaborative Biotechnologies through contract W911NF-19-D-0001 from the U.S. Army Research Office. The content of this report does not necessarily reflect the position or the policy of the Government, and no official endorsement should be inferred.
Funders:
Funding AgencyGrant Number
Army Research Office (ARO)W911NF-19-D-0001
Record Number:CaltechAUTHORS:20191202-140340910
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20191202-140340910
Official Citation:Construction of an inducible amyloid expression circuit in Bacillus megaterium: A case study with CsgA and TasA. Elin M. Larsson, John B. McManus, Richard M. Murray. bioRxiv 858266; doi: https://doi.org/10.1101/858266
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:100145
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:04 Dec 2019 18:46
Last Modified:04 Dec 2019 18:46

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