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Virus-transformed pre-B cells show ordered activation but not inactivation of immunoglobulin gene rearrangement and transcription

Schlissel, Mark S. and Corcoran, Lynn M. and Baltimore, David (1991) Virus-transformed pre-B cells show ordered activation but not inactivation of immunoglobulin gene rearrangement and transcription. Journal of Experimental Medicine, 173 (3). pp. 711-720. ISSN 0022-1007.

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Virus-transformed pre-B cells undergo ordered immunoglobulin (Ig) gene rearrangements during culture. We devised a series of highly sensitive polymerase chain reaction assays for Ig gene rearrangement and unrearranged Ig gene segment transcription to study both the possible relationship between these processes in cultured pre-B cells and the role played by heavy (H) chain (mu) protein in regulating gene rearrangement. Our analysis of pre-B cell cultures representing various stages of maturity revealed that transcription of each germline Ig locus precedes or is coincident with its rearrangement. Cell lines containing one functional rearranged H chain allele, however, continue to transcribe and to rearrange the allelic, unrearranged H chain locus. These cell lines appear to initiate but not terminate rearrangement events and therefore provide information about the requirements for activating rearrangement but not about allelic exclusion mechanisms.

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Baltimore, David0000-0001-8723-8190
Additional Information:Copyright © 1991 by Rockefeller University Press. Received for publication 19 October 1990 and in revised form 17 December 1990. We thank Drs. Peter Jackson and Tony DeFranco for comments on the manuscript and for many helpful discussions. We also thank Drs. N. Rosenberg and T. DeFranco, and Mr. A. Shapiro for providing cell lines. This work was supported by U.S. Public Health Service grant GM-39458. M.S. Schlissel is supported by a Bristol-Myers Cancer Research Fellowship. L.M. Corcoran is a Life Sciences Research Foundation Fellow.
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Deposited On:05 Apr 2008
Last Modified:03 Oct 2019 00:05

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