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Low- and high-thermogenic brown adipocyte subpopulations coexist in murine adipose tissue

Song, Anying and Dai, Wenting and Jang, Min Jee and Medrano, Leonard and Li, Zhuo and Zhao, Hu and Shao, Mengle and Tan, Jiayi and Li, Aimin and Ning, Tinglu and Miller, Marcia M. and Armstrong, Brian and Huss, Janice M. and Zhu, Yi and Liu, Yong and Gradinaru, Viviana and Wu, Xiwei and Jiang, Lei and Scherer, Philipp E. and Wang, Qiong A. (2020) Low- and high-thermogenic brown adipocyte subpopulations coexist in murine adipose tissue. Journal of Clinical Investigation, 130 (1). pp. 247-257. ISSN 0021-9738. PMCID PMC6934193. doi:10.1172/jci129167. https://resolver.caltech.edu/CaltechAUTHORS:20191203-132507070

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Abstract

Brown adipose tissue (BAT), as the main site of adaptive thermogenesis, exerts beneficial metabolic effects on obesity and insulin resistance. BAT has been previously assumed to contain a homogeneous population of brown adipocytes. Utilizing multiple mouse models capable of genetically labeling different cellular populations, as well as single-cell RNA sequencing and 3D tissue profiling, we discovered a new brown adipocyte subpopulation with low thermogenic activity coexisting with the classical high-thermogenic brown adipocytes within the BAT. Compared with the high-thermogenic brown adipocytes, these low-thermogenic brown adipocytes had substantially lower Ucp1 and Adipoq expression, larger lipid droplets, and lower mitochondrial content. Functional analyses showed that, unlike the high-thermogenic brown adipocytes, the low-thermogenic brown adipocytes have markedly lower basal mitochondrial respiration, and they are specialized in fatty acid uptake. Upon changes in environmental temperature, the 2 brown adipocyte subpopulations underwent dynamic interconversions. Cold exposure converted low-thermogenic brown adipocytes into high-thermogenic cells. A thermoneutral environment had the opposite effect. The recruitment of high-thermogenic brown adipocytes by cold stimulation is not affected by high fat diet feeding, but it does substantially decline with age. Our results revealed a high degree of functional heterogeneity of brown adipocytes.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1172/jci129167DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6934193/PubMed CentralArticle
ORCID:
AuthorORCID
Jang, Min Jee0000-0002-1536-7177
Medrano, Leonard0000-0002-0782-5473
Shao, Mengle0000-0002-5488-9904
Gradinaru, Viviana0000-0001-5868-348X
Scherer, Philipp E.0000-0003-0680-3392
Wang, Qiong A.0000-0003-2224-4287
Additional Information:© 2019 American Society for Clinical Investigation. Submitted: March 29, 2019; Accepted: September 25, 2019; Published: November 25, 2019. The authors are grateful to Jiandie Lin, Li Ye, and members of the Diabetes and Metabolism Research Institute for discussions and comments. The authors thank the City of Hope Animal Resource Center, Integrative Genomics Core, Electron Microscopy and Atomic Force Microscopy Core, Light Microscopy Core, Pathology (Solid Tumor) Core (supported by NIH P30CA033572), Analytical Cytometry Core, and City of Hope Comprehensive Cancer Center for guidance and assistance for experiments. This study was supported by NIH grants K01DK107788, R03HD095414, and R56AG063854 (to QAW) and R01DK55758, R01DK099110, P01DK088761, and P01AG051459 (to PES). QAW was also supported by City of Hope Caltech-COH Initiative Award and the American Diabetes Association Junior Faculty Development Award (1-19-JDF-023). PES was also supported by an unrestricted research grant from the Novo Nordisk Foundation and by a grant from the Kristian Gerhard Jebsen Foundation. This work was also supported by the Beckman Institute for CLARITY, Optogenetics and Vector Engineering Research for technology development and broad dissemination (http://clover.caltech.edu/) (to VG) and Caltech Divisional Postdoctoral Fellowship (to MJJ). Author Contributions: QAW, PES, and AS designed the experiments. QAW, PES, and LJ wrote the manuscript. AS and QAW handled all the mouse experiments and performed β-gal staining. AL performed histological sectioning. AS performed the mitochondrial membrane potential test and immunofluorescence staining. AS prepared primary brown adipocytes and XW conducted and analyzed scRNA-seq experiments. AS, QAW, LM, TN and WD performed the seahorse and fatty acid intake experiment. AS, MJJ, HZ, and BA performed BAT tissue clearing and 3D imaging. AS, ZL, and MMM performed the transmission electron microscopy. MS, JT, JMH, YL, YZ, LJ, and VG contributed to experimental design and discussion. All authors approved the final manuscript. The authors have declared that no conflict of interest exists.
Funders:
Funding AgencyGrant Number
NIHP30CA033572
NIHK01DK107788
NIHR03HD095414
NIHR56AG063854
NIHR01DK55758
NIHR01DK099110
NIHP01DK088761
NIHP01AG051459
Caltech-City of Hope Biomedical InitiativeUNSPECIFIED
American Diabetes Association1-19-JDF-023
Novo Nordisk FoundationUNSPECIFIED
Kristian Gerhard Jebsen FoundationUNSPECIFIED
Caltech Beckman InstituteUNSPECIFIED
Issue or Number:1
PubMed Central ID:PMC6934193
DOI:10.1172/jci129167
Record Number:CaltechAUTHORS:20191203-132507070
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20191203-132507070
Official Citation:Anying Song, … , Philipp E. Scherer, Qiong A. Wang Published January 2, 2020; First published October 1, 2019. J Clin Invest. 2020; 130(1): 247-257. https://doi.org/10.1172/JCI129167.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:100166
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:04 Dec 2019 18:12
Last Modified:16 Nov 2021 17:52

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