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Vein patterning by tissue-specific auxin transport

Govindaraju, Priyanka and Verna, Carla and Zhu, Tongbo and Scarpella, Enrico (2020) Vein patterning by tissue-specific auxin transport. Development, 147 (13). Art. No. dev187666. ISSN 0950-1991. https://resolver.caltech.edu/CaltechAUTHORS:20191209-135726053

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Abstract

Unlike in animals, in plants, vein patterning does not rely on direct cell-cell interaction and cell migration; instead, it depends on the transport of the plant hormone auxin, which in turn depends on the activity of the PIN-FORMED1 (PIN1) auxin transporter. The current hypotheses of vein patterning by auxin transport propose that, in the epidermis of the developing leaf, PIN1-mediated auxin transport converges to peaks of auxin level. From those convergence points of epidermal PIN1 polarity, auxin would be transported in the inner tissues where it would give rise to major veins. Here, we have tested predictions of this hypothesis and have found them unsupported: epidermal PIN1 expression is neither required nor sufficient for auxin transport-dependent vein patterning, whereas inner-tissue PIN1 expression turns out to be both required and sufficient for auxin transport-dependent vein patterning. Our results refute all vein patterning hypotheses based on auxin transport from the epidermis and suggest alternatives for future tests.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1242/dev.187666DOIArticle
https://doi.org/10.1101/866632DOIDiscussion Paper
https://dev.biologists.org/content/147/13/dev187666.supplementalPublisherSupplementary Information
ORCID:
AuthorORCID
Verna, Carla0000-0001-5312-5470
Scarpella, Enrico0000-0003-2962-0329
Additional Information:© 2020 Published by The Company of Biologists Ltd. Received 20 December 2019; Accepted 27 May 2020; Published 6 July 2020. We thank the Arabidopsis Biological Resource Center for PIN1::gPIN1:CFP, PIN1 cDNA and pin1-1; the Nottingham Arabidopsis Stock Centre for pin1-051; Ikram Blilou and Ben Scheres for pin3-3, pin4-2 and pin7^(En); Megan Sawchuk for PIN1::nYFP; and Jian Xu and Ben Scheres for PIN1::gPIN1:YFP and PIN1::gPIN1:GFP. This work was supported by Discovery Grants of the Natural Sciences and Engineering Research Council of Canada [RGPIN-2016-04736 to E.S.]. C.V. was supported, in part, by a University of Alberta Doctoral Recruitment Scholarship. Author contributions: Conceptualization: P.G., C.V., E.S.; Methodology: P.G., C.V., T.Z., E.S.; Validation: P.G., C.V., T.Z., E.S.; Formal analysis: P.G., C.V., T.Z., E.S.; Investigation: P.G., C.V., T.Z., E.S.; Writing - original draft: P.G., C.V., E.S.; Writing - review & editing: P.G., C.V., T.Z., E.S.; Visualization: P.G., C.V., T.Z., E.S.; Supervision: E.S.; Project administration: E.S.; Funding acquisition: E.S. The authors declare no competing or financial interests.
Funders:
Funding AgencyGrant Number
Natural Sciences and Engineering Research Council of Canada (NSERC)RGPIN-2016-04736
University of AlbertaUNSPECIFIED
Subject Keywords:Arabidopsis thaliana, Leaf development, Vascular patterning, Auxin, PIN genes, Necessity and sufficiency
Issue or Number:13
Record Number:CaltechAUTHORS:20191209-135726053
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20191209-135726053
Official Citation:Vein patterning by tissue-specific auxin transport. Priyanka Govindaraju, Carla Verna, Tongbo Zhu, Enrico Scarpella. Development 2020 147: dev187666 doi: 10.1242/dev.187666 Published 6 July 2020
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:100245
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:09 Dec 2019 22:04
Last Modified:24 Sep 2020 22:05

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