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Characterization of three isotypes of immunoglobulin light chains and T-cell antigen receptor α in zebrafish

Haire, Robert N. and Rast, Jonathan P. and Litman, Ronda T. and Litman, Gary W. (2000) Characterization of three isotypes of immunoglobulin light chains and T-cell antigen receptor α in zebrafish. Immunogenetics, 51 (11). pp. 915-923. ISSN 0093-7711. https://resolver.caltech.edu/CaltechAUTHORS:20191210-155234144

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Abstract

The zebrafish (Danio rerio) has become a significant model for understanding the developmental regulation of gene expression and holds considerable potential for characterizing the development of the immune system. Using a number of different approaches, including heterologous hybridization and short-primer PCR, cDNAs for three different classes of light-chain genes were identified and characterized. The zebrafish light chains are similar to trout type 1, trout type 2, and catfish type F, respectively. T-cell antigen receptor α (TCRα) was also identified and characterized. A high proportion of unusual transcripts including sterile transcripts, germline VJC transcripts, aberrant splice forms, and V-V transcripts were encountered in the immunoglobulin and TCR cDNAs examined. The light-chain and TCRα loci each consist of multiple families of V gene segments, apparent even from the small numbers of cDNAs of each isotype sequenced. The gene sequences reported provide an essential set of markers of both B- and T-cell lineages that will facilitate investigations of immune system development.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1007/s002510000229DOIArticle
https://rdcu.be/bYMt8PublisherFree ReadCube access
Additional Information:© 2000 Springer-Verlag. Received 30 March 2000; Revised 07 June 2000; Published online 8 August 2000. We would like to thank Barbara Pryor for editorial assistance. This work was supported by a grant to G.W.L. by the National Institutes of Health R37 AI23338.
Funders:
Funding AgencyGrant Number
NIHR37 AI23338
Subject Keywords:DNA sequence; development model; immunoglobulin diversity; short-primer PCR; teleost immunoglobulin
Issue or Number:11
Record Number:CaltechAUTHORS:20191210-155234144
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20191210-155234144
Official Citation:Haire, R., Rast, J., Litman, R. et al. Characterization of three isotypes of immunoglobulin light chains and T-cell antigen receptor α in zebrafish. Immunogenetics 51, 915–923 (2000) doi:10.1007/s002510000229
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:100271
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:11 Dec 2019 03:06
Last Modified:11 Dec 2019 03:06

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