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Sequential induction of NF-κB/Rel family proteins during B-cell terminal differentiation

Liou, Hsiou-Chi and Sha, William C. and Scott, Martin L. and Baltimore, David (1994) Sequential induction of NF-κB/Rel family proteins during B-cell terminal differentiation. Molecular and Cellular Biology, 14 (8). pp. 5349-5359. ISSN 0270-7306. PMCID PMC359054. https://resolver.caltech.edu/CaltechAUTHORS:LIOmcb94

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Abstract

The NF-kappa B/Rel family of at least five transcription factor polypeptides is thought to function both as a developmental regulator in B cells and as a rapid response system in all cells. To examine this notion in more detail, we determined the protein contents of both the inducible and constitutive NF-kappa B/Rel activities in a pre-B-cell line, 70Z/3, and a mature B-cell line, WEHI 231. NF-kappa B p50/p65 is the major inducible nuclear complex after lipopolysaccharide or phorbol myristate acetate treatment of 70Z/3 cells. The constitutive and inducible complexes in WEHI 231 cells are mainly composed of p50 and Rel. The constitutive or induced activities are all sensitive to I kappa B-alpha, but this inhibitor is very short-lived in WEHI 231 cells, suggesting that the balance between synthesis and degradation of I kappa B-alpha determines whether a particular cell lineage has constitutive activity. A patterned expression of the NF-kappa B/Rel activator proteins emerges from an analysis of other B-lineage cell lines and splenic B cells: mainly p50 and p65 in pre-B (and non-B) cells, a predominance of Rel and p50 in mature B cells, and expression of p52 and RelB in plasmacytoma lines. This ordered pattern of regulators may reflect the requirement for expression of different genes during terminal B-cell differentiation because different combinations of NF-kappa B/Rel family members preferentially activate distinct kappa B sites in reporter constructs.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1128/mcb.14.8.5349DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/pmc359054/PubMed CentralArticle
ORCID:
AuthorORCID
Baltimore, David0000-0001-8723-8190
Alternate Title:Sequential induction of NF-kappa B/Rel family proteins during B-cell terminal differentiation
Additional Information:© 1994 by the American Society for Microbiology. Received 22 March 1994/Returned for modification 25 April 1994/Accepted 6 May 1994. We thank R. Bravo, R. Dalla-Favera, N. Rice, and C.-C. Chang for the antisera against RelB and p52 proteins, and we thank L. Glimcher for M12 and p3X63Ag cell lines. We thank A. Czernik and P. Greengard for technical advice and facility on the phosphopeptide analyses. We also thank A. Beg, C. Roman, and Z.-S. Ye for critical comments on the manuscript. H.-C.L. is a Fellow of the Cancer Research Institute. W.S. is supported by a fellowship from NIH grant AI08724-02. This work, H.-C.L., and M.L.S. are supported by grants CA51462 and GM39458 to D.B.
Funders:
Funding AgencyGrant Number
Cancer Research InstituteUNSPECIFIED
NIHAI08724-02
NIHCA51462
NIHGM39458
Issue or Number:8
PubMed Central ID:PMC359054
Record Number:CaltechAUTHORS:LIOmcb94
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:LIOmcb94
Official Citation:Sequential induction of NF-kappa B/Rel family proteins during B-cell terminal differentiation. H C Liou, W C Sha, M L Scott, D Baltimore Molecular and Cellular Biology Aug 1994, 14 (8) 5349-5359; DOI: 10.1128/MCB.14.8.5349
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:10032
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:07 Apr 2008
Last Modified:23 Apr 2020 23:01

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