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A cryo–electron tomography workflow reveals protrusion-mediated shedding on injured plasma membrane

Mageswaran, Shrawan Kumar and Yang, Wei Yuan and Chakrabarty, Yogaditya and Oikonomou, Catherine M. and Jensen, Grant J. (2021) A cryo–electron tomography workflow reveals protrusion-mediated shedding on injured plasma membrane. Science Advances, 7 (13). Art. No. eabc6345. ISSN 2375-2548. PMCID PMC7997517. doi:10.1126/sciadv.abc6345. https://resolver.caltech.edu/CaltechAUTHORS:20200102-095248499

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Abstract

Cryo–electron tomography (cryo-ET) provides structural context to molecular mechanisms underlying biological processes. Although straightforward to implement for studying stable macromolecular complexes, using it to locate short-lived structures and events can be impractical. A combination of live-cell microscopy, correlative light and electron microscopy, and cryo-ET will alleviate this issue. We developed a workflow combining the three to study the ubiquitous and dynamic process of shedding in response to plasma membrane damage in HeLa cells. We found filopodia-like protrusions enriched at damage sites and acting as scaffolds for shedding, which involves F-actin dynamics, myosin-1a, and vacuolar protein sorting 4B (a component of the ‘endosomal sorting complex required for transport’ machinery). Overall, shedding is more complex than current models of vesiculation from flat membranes. Its similarities to constitutive shedding in enterocytes argue for a conserved mechanism. Our workflow can also be adapted to study other damage response pathways and dynamic cellular events.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1126/sciadv.abc6345DOIArticle
https://advances.sciencemag.org/content/suppl/2021/03/22/7.13.eabc6345.DC1PublisherSupplementary Materials
https://doi.org/10.1101/2019.12.23.887638DOIDiscussion Paper
http://www.ncbi.nlm.nih.gov/pmc/articles/pmc7997517/PubMed CentralArticle
ORCID:
AuthorORCID
Chakrabarty, Yogaditya0000-0002-3230-9251
Oikonomou, Catherine M.0000-0003-2312-4746
Jensen, Grant J.0000-0003-1556-4864
Alternate Title:Plasma membrane damage removal by F-actin-mediated shedding from repurposed filopodia
Additional Information:© 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). Submitted 5 May 2020; Accepted 9 February 2021; Published 26 March 2021. We thank A. A. Hyman and I. Poser for providing the HeLa cell line stably expressing CHMP4B-EGFP. We thank A. Collazo and S. Wilbert for technical assistance with confocal microscopy. We also thank S. Chen and A. Malyutin for technical assistance with cryo-EM. The bulk of the confocal imaging was performed at the Biological Imaging Facility, and EM was performed at the Beckman Institute Resource Center for Transmission Electron Microscopy, both at Caltech. Funding: This work was supported by funding from the NIH (P50 AI150464 awarded to G.J.J.). Author contributions: W.Y.Y. performed light microscopy experiments and prepared sample for cryo-ET with help from S.K.M. S.K.M. performed CLEM, cryo-ET, and data analyses. S.K.M. prepared the manuscript with help from W.Y.Y., C.M.O., and G.J.J. Y.C. performed the Western blot assays for Myo1a and Vps4B knockdowns. Competing interests: The authors declare that they have no competing interests. Data and materials availability: All data needed to evaluate the conclusions in the paper are present in the paper and/or the Supplementary Materials. Additional data related to this paper may be requested from the first and corresponding authors.
Funders:
Funding AgencyGrant Number
NIHP50 AI150464
Issue or Number:13
PubMed Central ID:PMC7997517
DOI:10.1126/sciadv.abc6345
Record Number:CaltechAUTHORS:20200102-095248499
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200102-095248499
Official Citation:S. K. Mageswaran, W. Y. Yang, Y. Chakrabarty, C. M. Oikonomou, G. J. Jensen, A cryo–electron tomography workflow reveals protrusion-mediated shedding on injured plasma membrane. Sci. Adv. 7, eabc6345 (2021)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:100447
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:02 Jan 2020 19:38
Last Modified:19 Apr 2021 21:27

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