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Molecular hybridization used to characterize the RNA synthesized by isolated bovine thymus nuclei

Sullivan, David T. (1968) Molecular hybridization used to characterize the RNA synthesized by isolated bovine thymus nuclei. Proceedings of the National Academy of Sciences of the United States of America, 59 (3). pp. 846-853. ISSN 0027-8424.

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In vitro synthesis of RNA by nuclei isolated from various tissues has been observed in many laboratories (1-3). Several investigators have realized the usefulness of isolated nuclei for studying the regulation of RNA synthesis. The effects of nuclear proteins (4) and various hormones, (5-7) for example, on the incorporation of various precursors into RNA have been analyzed recently. However, if the full potential of isolated nuclei as an assay system for studying the regulation of RNA synthesis is to be realized, then it is necessary that the characterization of the synthesized RNA be more complete. In the present studies, we have used the technique of DNA-RNA hybridization in an attempt to characterize the RNA synthesized in vitro by thymus nuclei. We have shown that the isolated thymus nucleus is capable of synthesizing a population of RNA molecules that is specific to thymus. The intracellular distribution of RNA species was also investigated and differences were found between nuclear RNA and cytoplasmic RNA. The isolated nucleus was shown to synthesize RNA similar to that found in the nucleus and in the cytoplasm.

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Additional Information:© 1968 by the National Academy of Sciences. Communicated by Clement L. Markert, January 8, 1968. Submitted to The Johns Hopkins University in partial fulfillment of the requirements for the degree of Doctor of Philosophy. Work supported by predoctoral training grant 1 T1 HD-139-01 and NSF research grant GB-4451. The author acknowledges the advice of Dr. H. Ursprung during the course of this work. The helpful suggestions of Dr. K. Smith are also gratefully acknowledged.
Issue or Number:3
Record Number:CaltechAUTHORS:SULpnas68
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:10045
Deposited By: Tony Diaz
Deposited On:08 Apr 2008
Last Modified:03 Oct 2019 00:06

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